A large cluster of highly expressed genes is dispensable for growth and development in Aspergillus nidulans.
A large cluster of highly expressed genes is dispensable for growth and development in Aspergillus nidulans.
复制标题
构巢曲霉的生长和发育离不开大量高表达基因。
作者：
R. Aramayo;T. Adams;W. Timberlake
我们通过删除整个38kb的SpoC1基因簇，研究了高表达的、芽胞形成特异性的Nidulans SpoC1基因的功能。突变株与野生型在(1)生长速度、(2)在完成无性或有性生活周期期间形成的特化生殖结构的形态、(3)产孢率、(4)孢子活力或(5)对环境胁迫的抵抗力方面没有不同。因此，SpoC1基因簇的缺失，占纽假单胞菌基因组的0.15%，没有容易检测到的表型效应。这一结果的含义在发生在弧菌发育过程中的基因表达的主要变化的背景下进行了讨论。
We investigated the functions of the highly expressed, sporulation-specific SpoC1 genes of Aspergillus nidulans by deleting the entire 38-kb SpoC1 gene cluster. The resultant mutant strain did not differ from the wild type in (1) growth rate, (2) morphology of specialized reproductive structures formed during completion of the asexual or sexual life cycles, (3) sporulation efficiency, (4) spore viability or (5) spore resistance to environmental stress. Thus, deletion of the SpoC1 gene cluster, representing 0.15% of the A. nidulans genome, had no readily detectable phenotypic effects. Implications of this result are discussed in the context of major alterations in gene expression that occur during A. nidulans development.