Polyelectrolyte complex membranes for specific cell adhesion
Polyelectrolyte complex membranes for specific cell adhesion
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DOI：
10.1021/la7025768
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发表时间：
2008-03-18
期刊：
影响因子：
3.9
通讯作者：
Ying, Jackie Y.
中科院分区：
文献类型：
--
作者：
Wan, Andrew C. A.;Tai, Benjamin C. U.;Ying, Jackie Y.
生物活性配体在生物材料表面的呈现经常被存在于生物环境中的蛋白质的吸附所混淆，使得任何类型的细胞反应都是非特异性的。我们设计了一种聚电解质复合膜，它展示了二维(2D)和三维(3D)细胞培养系统中各种细胞类型的特定粘附性。细胞黏附是通过三层结构实现的：二氧化硅交联聚阳离子作为底层(第一层)，无污染聚阴离子-聚乙二醇络合物作为中间层(第二层)，细胞黏附配体作为上层(第三层)。根据化学成分和尺寸对膜的每一层进行了表征。在膜上培养的上皮细胞(原代人肾皮质细胞和肝癌细胞系)在聚阴离子-聚乙二醇二层上几乎没有细胞附着，而在RGD修饰的第三层上细胞附着良好。因此，第二层允许由于配体(第三层)引起的细胞黏附效应被分离出来，并与第一层的非特异性细胞黏附区分开来。对于细胞的三维培养，采用三层膜系统，使用高度膨胀的壳聚糖膜作为第一层。得到的细胞膜结构被均匀分散和离心以形成基质，该基质以颗粒的形式与细胞密切相互作用。与没有RGD的对照组相比，后一种形式的RGD表达增强了人骨髓间充质干细胞(HMSCs)的活性。
The presentation of bioactive ligands on biomaterial surfaces is often confounded by the adsorption of proteins present in the biological milieu, rendering any type of cellular response nonspecific. We have engineered a polyelectrolyte complex membrane that demonstrates specific adhesion of various cell types for both two-dimensional (2D) and three-dimensional (3D) cell culture systems. Specific cell adhesion is achieved by a three-tiered structure: a silica cross-linked polycation as the bottom (first) tier, a nonfouling polyanion-poly(ethylene glycol) (PEG) conjugate as the intermediate (second) tier, and the cell-adhesion ligand as the top (third) tier. Each tier of the membrane was characterized in terms of chemical composition and dimensions. Epithelial cells (primary human cortical renal cells and a hepatocellular carcinoma cell line) cultured on the membranes exhibited little cell attachment on the polyanion - PEG second tier and good cell adhesion on the RGD-modified third tier. Thus, the second tier allowed the effect of cell adhesion due to the ligand (third tier) to be isolated and distinguished from nonspecific cell attachment to the first tier. For the culturing of cells in three dimensions, the three-tiered membrane system was applied using a highly swellable chitosan membrane as the first tier. The resulting cell-membrane construct was uniformly dispersed and centrifuged to form a matrix that interacted intimately with cells in the form of a pellet. Presentation of RGD in the latter format enhanced the viability of human mesenchymal stem cells (hMSCs) over controls without RGD.