Biodegradation of (1→3)-β-polyglucuronate prepared by TEMPO-mediated oxidation.
Biodegradation of (1→3)-β-polyglucuronate prepared by TEMPO-mediated oxidation.
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DOI：
10.1016/j.carbpol.2013.03.081
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发表时间：
2013-07
影响因子：
11.2
通讯作者：
É. Watanabe;N. Habu;A. Isogai
中科院分区：
文献类型：
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作者：
É. Watanabe;N. Habu;A. Isogai
采用2，2，6，6-四甲基哌啶-1-氧自由基（克里思）氧化可得然胶制备水溶性（1→3）-β-聚葡萄糖醛酸钠，并研究其生物降解性能。从土壤中分离到一株能降解（1→3）-β-聚葡萄糖醛酸的菌株（EH 621），经鉴定为类芽孢杆菌属（Paenibacillus sp.）。表明（1→3）-β-聚葡萄糖醛酸可以容易地被自然环境中存在的微生物降解和代谢。在EH 621的无细胞提取物中检测到水解酶活性，该酶对（1→3）-β-聚葡萄糖醛酸具有高度特异性。对酶降解产物的分析表明，（1→3）-β-聚葡萄糖醛酸通过内水解途径降解为葡萄糖醛酸单体。
2,2,6,6-Tetramethylpiperidine-1-oxyl radical (TEMPO)-mediated oxidation was applied to curdlan to prepare water-soluble sodium (1→3)-β-polyglucuronate, and its biodegradation behavior was then investigated. A bacterial strain (EH621) having the ability to degrade (1→3)-β-polyglucuronate was isolated from soil, and identified as Paenibacillus sp. Strain EH621 cultured with (1→3)-β-polyglucuronate decreased the initial total carbon in the supernatant by approximately 60% within 3 days, showing that (1→3)-β-polyglucuronate can be readily degraded and metabolized by microorganisms present in the natural environment. Hydrolytic enzyme activity was detected in the cell-free extract of EH621, which was highly specific to (1→3)-β-polyglucuronate. Analyses of the enzymatic degradation products revealed that (1→3)-β-polyglucuronate was endolytically degraded to its monomeric unit, glucuronate, via some oligomers and dimers.