GABA AND ENKEPHALIN PROJECTION FROM THE NUCLEUS-ACCUMBENS AND VENTRAL PALLIDUM TO THE VENTRAL TEGMENTAL AREA
GABA AND ENKEPHALIN PROJECTION FROM THE NUCLEUS-ACCUMBENS AND VENTRAL PALLIDUM TO THE VENTRAL TEGMENTAL AREA
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DOI：
10.1016/0306-4522(93)90048-k
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发表时间：
1993-12-01
期刊：
影响因子：
3.3
通讯作者：
KLITENICK, MA
中科院分区：
文献类型：
--
作者：
KALIVAS, PW;CHURCHILL, L;KLITENICK, MA
用逆行追踪技术结合原位杂交技术研究了大鼠腹侧被盖区的GABA能和脑啡肽能传入。继腹侧被盖区电泳金沉积后，前脑标记最密集的部位是伏核外壳、苍白球头端腹内侧腹侧腹侧苍白球和Broca斜角带。外侧隔区的密度也较小。在这些前脑区，含有谷氨酸脱羧酶基因的逆行标记细胞的比例从25%到50%不等，而前脑啡肽原基因的双标记细胞仅占5%到15%。谷氨酸脱羧酶或前脑啡肽原基因双标细胞主要分布在外侧隔核、伏隔核壳、苍白球吻侧腹侧和Broca斜角带。大量的荧光金沉积侵入黑质内侧，导致伏隔核核心内有大量逆标细胞，部分神经元还含有谷氨酸脱羧酶或前脑啡肽原的mRNA。这些结果表明，GABA能和脑啡肽能神经元从伏隔核、腹侧苍白球和斜角带投射到腹侧被盖区。
GABAergic and enkephalinergic afferents to the ventral tegmental area were investigated in the rat using retrograde tracing techniques combined with in situ hybridization. Following iontophoretic deposit of Fluoro-Gold in the ventral tegmental area labeling in the forebrain was most dense in the shell of the nucleus accumbens, rostral ventromedial ventral pallidum and diagonal band of Broca. A smaller density was also observed in the lateral septum. In these forebrain regions, the portion of retrogradely labeled cells that contained mRNA for glutamate decarboxylase ranged from 25% to 50%, whereas only 5% to 15% were double-labeled for preproenkephalin mRNA. Cells double-labeled with either glutamate decarboxylase or preproenkephalin mRNA were most numerous in the lateral septum, shell of the nucleus accumbens, rostral ventral pallidum and diagonal band of Broca. Large Fluoro-Gold deposits which invaded the medial substantia nigra resulted in a significant number of retrogradely labeled cells in the core of the nucleus accumbens, and a portion of these neurons also contained mRNA for glutamate decarboxylase or preproenkephalin.These data demonstrate the presence of GABAergic and enkephalinergic neurons projecting from the nucleus accumbens, ventral pallidum and diagonal band of Broca to the ventral tegmental area.