A selective agar medium for isolation, enumeration and morphological identification of Fusarium proliferatum
A selective agar medium for isolation, enumeration and morphological identification of Fusarium proliferatum
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DOI：
10.1007/s12600-014-0392-7
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发表时间：
2014-09-01
期刊：
影响因子：
1.4
通讯作者：
Gamliel, A.
中科院分区：
文献类型：
--
作者：
Isack, Yochai;Benichis, Marina;Gamliel, A.
以枣浸渍果实为基础，开发了一种选择性琼脂培养基，用于从土壤和各种植物（包括洋葱鳞茎、玉米穗和茎以及各种杂草组织）的感染组织中分离、枚举和形态鉴定增殖镰刀菌。选择性枣培养基促进了多酚化物的形成和更长的分生孢子链，以便更好地与其他相关的镰刀菌分离，这些镰刀菌也在该培养基上生长和增殖。此外，枣培养基可以在显微镜下区分其他密切相关的镰刀菌，例如f.s oxysporum和f.s verticillioides。与其他品种相比，枣品种Medjoul和Deglet Noor的果实提供了最有用的结果。枣培养基可以作为直接分离和枚举增殖酸乳杆菌的选择性培养基，因为它可以抑制其他土壤真菌和植物病原体（如phaseolina Macrophomina， Sclerotium rolfsii和Rhizoctonia solani）以及细菌的发育。
A selective agar medium based on macerated date fruits was developed for the isolation, enumeration and morphological identification of Fusarium proliferatum from soil and from infected tissues of various plants (including: onion bulbs, corn ears and stems, and various weed tissues). The selective date medium enhances the formation of polyphialide and longer chains of conidia for better separation from other related Fusarium species which also grow and proliferate on this medium. Furthermore, the date medium enables microscopic distinction among other closely related Fusarium species, e.g. F. oxysporum and F. verticillioides. Fruits of the date cultivars Medjoul and Deglet Noor provided the most useful results as compared with other cultivars tested. The date medium can serve as a selective medium for direct isolation and enumeration of F. proliferatum, as it suppresses the development of other soil fungi and plant pathogens such as Macrophomina phaseolina, Sclerotium rolfsii and Rhizoctonia solani, as well as bacteria.