Roles of intracellular hydrogen peroxide accumulation in abscisic acid signaling in Arabidopsis guard cells.
Roles of intracellular hydrogen peroxide accumulation in abscisic acid signaling in Arabidopsis guard cells.
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DOI：
10.1016/j.jplph.2011.05.006
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发表时间：
2011-11-01
影响因子：
4.3
通讯作者：
Murata, Yoshiyuki
中科院分区：
文献类型：
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作者：
Jannat, Rayhanur;Uraji, Misugi;Morofuji, Miho;Islam, Mohammad Muzahidul;Bloom, Rachel E;Nakamura, Yoshimasa;McClung, C Robertson;Schroeder, Julian I;Mori, Izumi C;Murata, Yoshiyuki
活性氧（Reactive oxygen species，ROS），包括过氧化氢（hydrogen peroxide，H2 O2），是保卫细胞中脱落酸（abscisic acid，阿坝）信号转导的重要第二信使。在这项研究中，探讨特定的角色，过氧化氢在保卫细胞中的阿坝信号，我们研究了突变的保卫细胞表达的过氧化氢酶（CAT）基因，CAT 1和CAT 3，和CAT抑制剂3-氨基三唑（AT）对气孔运动的影响。cat 3和cat 1 cat 3突变显著降低CAT活性，导致保卫细胞中H2 O2的基础水平升高，但对非胁迫条件下气孔孔径大小无影响。此外，在抑制CAT活性的浓度下，AT处理对气孔孔径大小的影响不大，而基础H_2O_2水平则显著增加。相反，cat突变和AT处理增强ABA诱导的气孔关闭。阿坝诱导的ROS产生触发观察到在这些突变体和野生型以及AT处理的保卫细胞。这些结果表明，ABA诱导的胞质H_2O_2升高在ABA诱导的气孔关闭中起作用，而H_2O_2的组成性升高不引起气孔关闭。
Reactive oxygen species (ROS), including hydrogen peroxide (H2O2), are among the important second messengers in abscisic acid (ABA) signaling in guard cells. In this study, to investigate specific roles of H2O2 in ABA signaling in guard cells, we examined the effects of mutations in the guard cell-expressed catalase (CAT) genes, CAT1 and CAT3, and of the CAT inhibitor 3-aminotriazole (AT) on stomatal movement. The cat3 and cat1 cat3 mutations significantly reduced CAT activities, leading to higher basal level of H2O2 in guard cells, when assessed by 2′,7′-dichlorodihydrofluorescein, whereas they did not affect stomatal aperture size under non-stressed condition. In addition, AT-treatment at the concentrations that abolish CAT activities, showed trivial affect on stomatal aperture size, while basal H2O2 level increased extensively. In contrast, cat mutations and AT-treatment potentiated ABA-induced stomatal closure. Inducible ROS production triggered by ABA was observed in these mutants and wild type as well as in AT-treated guard cells. These results suggest that the ABA-inducible cytosolic H2O2 elevation functions in ABA-induced stomatal closure, while constitutive raise of H2O2 did not caused stomatal closure.