Non-side-population hematopoietic stem cells in mouse bone marrow

Non-side-population hematopoietic stem cells in mouse bone marrow
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DOI：
10.1182/blood-2006-03-010207
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发表时间：
2006-10-15
期刊：
BLOOD
影响因子：
20.3
通讯作者：
Nakauchi, Hiromitsu
Nakauchi, Hiromitsu
中科院分区：
医学1区
文献类型：
--
作者：
Morita, Yohei;Ema, Hideo;Nakauchi, Hiromitsu

文献摘要

被引文献

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大多数造血干细胞 (HSC) 被认为存在于成年小鼠骨髓 (BM) 中所谓的侧群 (SP) 中。我们报告了非 SP HSC 的共存，这些非 SP HSC 在数量、容量和细胞周期状态方面与 SP HSC 没有显着差异。当用 Hoechst 33342 染料染色时，小鼠 HSC 中高度富集的 CD34(-/low) c-Kit(+)Sca(-1+) 谱系标记 (-) (CD34(-)KSL) 细胞群几乎均等地分为 SP 和代表非 SP 细胞的主要细胞群 (MP)。使用单个或 30 个 SP-或 MP-CD34(-)KSL 细胞进行的竞争性再增殖测定发现，这些细胞群的再增殖活性和再增殖细胞频率相似。二次移植检测了两个群体的自我更新能力。对原代受体小鼠的 BM 细胞进行 SP 分析表明 SP 和 MP 表型是可以相互转换的。细胞周期分析显示，无论SP还是MP，CD34(-)KSL细胞均处于静止状态，并表现出一致的细胞周期动力学。在SP-和MP-CD34(-)KSL细胞中，Bcrp-1的表达相似，表明SP表型不仅受Bcrp-1调控，还受其他因素的调控。 SP 表型并不指定所有 HSC；因此它与干细胞功能的同一性是不可能的。
Most hematopoietic stem cells (HSCs) are assumed to reside in the so-called side population (SP) in adult mouse bone marrow (BM). We report the coexistence of non-SP HSCs that do not significantly differ from SP HSCs in numbers, capacities, and cell-cycle states. When stained with Hoechst 33342 dye, the CD34(-/low) c-Kit(+)Sca(-1+) lineage marker(-) (CD34(-)KSL) cell population, highly enriched in mouse HSCs, was almost equally divided into the SP and the main population (MP) that represents non-SP cells. Competitive repopulation assays with single or 30 SP-or MP-CD34(-)KSL cells found similar degrees of repopulating activity and frequencies of repopulating cells for these populations. Secondary transplantation detected self-renewal capacity in both populations. SP analysis of BM cells from primary recipient mice suggested that the SP and MP phenotypes are interconvertible. Cell-cycle analyses revealed that CD34(-)KSL cells were in a quiescent state and showed uniform cell-cycle kinetics, regardless of whether they were in the SP or MP Bcrp-1 expression was similarly detected in SP- and MP-CD34(-)KSL cells, suggesting that the SP phenotype is regulated not only by Bcrp-1, but also by other factors. The SP phenotype does not specify all HSCs; its identity with stem cell function thus is unlikely.