Leukemic T cells are specifically enriched in a unique CD3dimCD7low subpopulation of CD4+ T cells in acute-type adult T-cell leukemia
Leukemic T cells are specifically enriched in a unique CD3dimCD7low subpopulation of CD4+ T cells in acute-type adult T-cell leukemia
复制标题
DOI：
10.1111/j.1349-7006.2010.01833.x
复制
发表时间：
2011-03-01
期刊：
影响因子：
5.7
通讯作者：
Uchimaru, Kaoru
中科院分区：
文献类型：
--
作者：
Tian, Yamin;Kobayashi, Seiichro;Uchimaru, Kaoru
在成人T细胞白血病（ATL）中，白血病T细胞与非白血病T细胞的形态区分通常很困难，因为ATL细胞显示出形态多样性，除了典型的“花细胞”。“由于ATL细胞中CD 3和CD 7表达的缺陷很常见，我们应用多色流式细胞术检测ATL患者外周血中假定的白血病特异性细胞群。根据CD 3 vs CD 7图对CD 4（+）CD 14（-）细胞进行双色分析，清楚地表明9例急性型ATL患者中均存在CD 3（dim）CD 7（low）亚群。来自该级分的大多数分选的细胞显示花细胞样形态，并携带高的人T细胞白血病病毒1型（HTLV-I）前病毒载量。基因组整合位点分析（反向长程PCR）和T细胞受体V β库的流式细胞术分析表明，大多数白血病细胞包括在CD 3（低）CD 7（低）亚群。这些结果表明，白血病T细胞特异性富集在一个独特的CD 3（低）CD 7（低）亚群的CD 4（+）T细胞在急性型ATL。（Cancer Sci 2011; 102：569-577）
The morphological discrimination of leukemic from non-leukemic T cells is often difficult in adult T-cell leukemia (ATL) as ATL cells show morphological diversity, with the exception of typical "flower cells." Because defects in the expression of CD3 as well as CD7 are common in ATL cells, we applied multi-color flow cytometry to detect a putative leukemia-specific cell population in the peripheral blood from ATL patients. CD4(+)CD14(-) cells subjected to two-color analysis based on a CD3 vs CD7 plot clearly demonstrated the presence of a CD3(dim)CD7(low) subpopulation in each of nine patients with acute-type ATL. The majority of sorted cells from this fraction showed a flower cell-like morphology and carried a high proviral load for the human T-cell leukemia virus type 1 (HTLV-I). Genomic integration site analysis (inverse long-range PCR) and analysis of the T cell receptor V beta repertoire by flow cytometry indicated that the majority of leukemia cells were included in the CD3(dim)CD7(low) subpopulation. These results suggest that leukemic T cells are specifically enriched in a unique CD3(dim)CD7(low) subpopulation of CD4(+) T cells in acute-type ATL. (Cancer Sci 2011; 102: 569-577)