WIDESPREAD PRESENCE OF HISTOLOGICALLY OCCULT CYTOMEGALOVIRUS
WIDESPREAD PRESENCE OF HISTOLOGICALLY OCCULT CYTOMEGALOVIRUS
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DOI：
10.1016/s0046-8177(84)80076-3
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发表时间：
1984-01-01
期刊：
影响因子：
3.3
通讯作者：
MCDOUGALL, JK
中科院分区：
文献类型：
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作者：
MYERSON, D;HACKMAN, RC;MCDOUGALL, JK
在福尔马林固定的石蜡包埋的组织切片中，用生物素标记的DNA探针进行原位杂交，以检测播散性巨细胞病毒(CMV)。用这种高度特异的技术对两例播散性巨细胞病毒感染的尸检进行了研究。巨细胞病毒在巨细胞中的存在很容易被显示出来。此外，在许多外观正常的细胞中也检测到并定位了CMV。这种隐匿性感染发生在心肌细胞、肝细胞、脾和淋巴结网状细胞、子宫内膜间质和腺细胞和乳腺间质细胞，以及肾小球、肾小管和间质、肾上腺皮质和髓质、输卵管粘膜下层、子宫肌层和垂体前叶的细胞中。利用抗第VIII因子抗体的免疫组织化学方法进一步证实了巨细胞病毒对内皮细胞的感染。巨细胞病毒明显地通过血液在全身传播，启动了坏死灶的感染。许多弥漫性病灶的出现表明局部病毒通过内皮细胞感染而传播。令人惊讶的是，没有观察到淋巴细胞的参与。
Disseminated cytomegalovirus (CMV) was investigated by in situ hybridization in formalin-fixed paraffin-embedded tissue sections with biotinylated DNA probes. Two cases [human] of disseminated CMV infection were studied at autopsy by this highly specific technique. The presence of CMV in cytomegalic cells is readily shown. In addition, CMV was detected and localized in many normal-appearing cells. This occult infection occurs in cardiac myocytes, hepatocytes, spleen and lymph node reticular cells, endometrial stromal and glandular cells and breast stromal cells, as well as in cells in the renal glomerulus, tubule and interstitium, adrenal cortex and medulla, fallopian tube submucosa, myometrium and anterior pituitary. Cytomegalovirus infection of endothelial cells was further documented by immunohistochemical methods utilizing antibody to Factor VIII. CMV evidently disseminates hematogenously throughout the body, initiating necrotizing foci of infection. The appearance of many diffuse foci suggests that local viral spread occurs via endothelial cell infection. Surprisingly, lymphocyte involvement was not observed.