A COMMON NEUTRALIZING EPITOPE CONSERVED BETWEEN THE HEMAGGLUTININS OF INFLUENZA-A VIRUS H1 AND H2 STRAINS
A COMMON NEUTRALIZING EPITOPE CONSERVED BETWEEN THE HEMAGGLUTININS OF INFLUENZA-A VIRUS H1 AND H2 STRAINS
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DOI：
10.1128/jvi.67.5.2552-2558.1993
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发表时间：
1993-05-01
影响因子：
5.4
通讯作者：
UEDA, S
中科院分区：
文献类型：
--
作者：
OKUNO, Y;ISEGAWA, Y;UEDA, S
当用流感病毒A/Okuda/57（H2 N2）株免疫小鼠时，获得了命名为C179的独特单克隆抗体。尽管通过免疫沉淀试验证实C179识别血凝素（HA）糖蛋白，但其对甲型流感病毒的三种亚型的任何毒株均未显示血凝抑制活性。然而，它中和了所有的H1和H2菌株，但不中和H3菌株。此外，它还能抑制H1和H2菌株诱导的多核体形成，但对H3菌株无抑制作用。对两个抗C179的抗原变异体进行了核苷酸序列分析，结果表明，H1和H2毒株之间保守的HA 1的318 - 322和HA 2的47 - 58的氨基酸序列负责识别C179。由于这两个位点在HA分子的茎区的中间彼此靠近，C179似乎在构象上识别这些位点。这些数据表明，C179与HA的茎区的结合抑制了HA的融合活性，从而导致病毒中和和细胞-细胞融合的抑制。这是第一份报告，描述了保守的抗原位点的HA不仅在一个特定的亚型，但也在两个亚型的流感病毒A。
When mice were immunized with the A/Okuda/57 (H2N2) strain of influenza virus, a unique monoclonal antibody designated C179 was obtained. Although C179 was confirmed to recognize the hemagglutinin (HA) glycoprotein by immunoprecipitation assays, it did not show hemagglutination inhibition activity to any of the strains of the three subtypes of influenza A virus. However, it neutralized all of the H1 and H2 strains but not the H3 strains. Moreover, it inhibited polykaryon formation induced by the H1 and H2 strains but not by the H3 strains. Two antigenic variants against C179 were obtained, and nucleotide sequence analysis revealed that amino acid sequences, from 318 to 322 of HA1 and from 47 to 58 of HA2, conserved among H1 and H2 strains were responsible for the recognition of C179. Since the two sites were located close to each other at the middle of the stem region of the HA molecule, C179 seemed to recognize these sites conformationally. These data indicated that binding of C179 to the stem region of HA inhibits the fusion activity of HA and thus results in virus neutralization and inhibition of cell-cell fusion. This is the first report which describes the presence of conserved antigenic sites on HA not only in a specific subtype but also in two subtypes of influenza A virus.