Noradrenaline transport and transporter mRNA of rat chromaffin cells are controlled by dexamethasone and nerve growth factor.
Noradrenaline transport and transporter mRNA of rat chromaffin cells are controlled by dexamethasone and nerve growth factor.
复制标题
大鼠嗜铬细胞的去甲肾上腺素转运和转运蛋白 mRNA 受地塞米松和神经生长因子控制。
DOI：
10.1113/jphysiol.1996.sp021476
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Bannon,MJ
中科院分区：
文献类型：
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作者：
Wakade,AR;Wakade,TD;Poosch,M;Bannon,MJ
1. 研究了肾上腺髓质中的嗜铬细胞与原代培养中的嗜铬细胞之间去甲肾上腺素（NA）转运蛋白功能差异的生化基础。 2.新生大鼠完整的肾上腺髓质积聚少量[3H]NA。相比之下，培养 2-6 天的解离嗜铬细胞积累的 [3H]NA 增加了 100-1000 倍。 3.神经生长因子（NGF）被刺激，而糖皮质激素剂量依赖性且可逆地抑制嗜铬细胞中的[3H]NA转运，该转运在培养中维持长达6天。在此期间，NGF 处理或未处理的嗜铬细胞的形态或生化特征没有明显变化。 4.分离大鼠NA转运蛋白cDNA克隆用于NA转运蛋白mRNA的定量。完整的肾上腺髓质所含的 NA 转运蛋白 mRNA 比培养中同等数量的嗜铬细胞少 40%。此外，地塞米松导致培养细胞中 NA 转运蛋白 mRNA 水平减少近 90%，而 NGF 导致 NA 转运蛋白 mRNA 水平增加约 60%。 5.在培养的细胞中，并且可能在体内，糖皮质激素至少部分地通过减少NA转运蛋白mRNA来抑制嗜铬细胞的NA转运蛋白功能。
1. The biochemical basis for differences in noradrenaline (NA) transporter function between chromaffin cells in the adrenal medulla and those maintained in primary culture was investigated. 2. Intact adrenal medullae of neonatal rats accumulated small amounts of [3H]NA. In contrast, dissociated chromaffin cells placed in culture for 2‐6 days accumulated 100‐1000 times more [3H]NA. 3. Nerve growth factor (NGF) stimulated, whereas glucocorticoids dose dependently and reversibly inhibited, [3H]NA transport in chromaffin cells maintained in culture up to 6 days. During this period, no change in the morphological or biochemical characteristics of either NGF‐treated or ‐untreated chromaffin cells was evident. 4. A rat NA transporter cDNA clone was isolated for use in the quantification of NA transporter mRNA. Intact adrenal medullae contained 40% less NA transporter mRNA than an equivalent number of chromaffin cells in culture. Furthermore, dexamethasone produced nearly 90% loss and NGF elicited approximately 60% increase in NA transporter mRNA levels in cultured cells. 5. In cultured cells, and possibly in vivo, glucocorticoids inhibit NA transporter function of chromaffin cells at least in part through a decrease in NA transporter mRNA.