Cis-lycopene is more bioavailable than trans-lycopene in vitro and in vivo in lymph-cannulated ferrets
Cis-lycopene is more bioavailable than trans-lycopene in vitro and in vivo in lymph-cannulated ferrets
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DOI：
10.1093/jn/129.6.1176
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发表时间：
1999-06-01
影响因子：
4.2
通讯作者：
Erdman, JW
中科院分区：
文献类型：
--
作者：
Boileau, AC;Merchen, NR;Erdman, JW
番茄红素是番茄和番茄基食品中的主要类胡萝卜素，也是人体血清和组织中的主要类胡萝卜素。最近，摄入富含番茄红素的食物与降低患几种慢性病的风险有关。血清和组织中的番茄红素含有50%以上的顺式番茄红素，而番茄和以番茄为主的食品中主要含有全反式番茄红素，这导致了番茄红素的顺式异构体更具生物利用度的假说。我们在体外(研究1)和体内(研究2)检验了这一假设。在研究1中，制备了含有结晶番茄红素的胆汁酸胶束。用于这些分析的结晶番茄红素是54.4%的顺式番茄红素。经反复分析，光学透明胶束制剂中含有75.9%的顺式番茄红素。在研究2中，利用肠系膜淋巴管插管雪貂研究了Lycored(TM)(番茄的乙酸乙酯提取物，含有5%的番茄红素，其中91%是全反式番茄红素)对番茄红素的体内吸收。在麻醉前，雄性雪貂(n=7)在大豆油中按每公斤体重40 mg番茄红素灌胃。冰冻2 h收集淋巴分泌物，用高效液相色谱法分析残胃和小肠内容物、粘膜衬里、淋巴分泌物和血清。剂量、胃和肠内容物中顺式番茄红素含量为6.2-17.5%，肠系膜淋巴分泌物中顺式番茄红素含量为77.4%(P<；0.01)。这些研究表明，在雪貂体内，番茄红素的顺式异构体比反式番茄红素具有更高的生物利用度，这可能是因为顺式异构体在胆汁酸胶束中更容易溶解，并可能优先结合到乳糖粒中。
Lycopene is the predominant carotenoid in tomatoes and tomato-based foods and is also a predominant carotenoid in human serum and tissues. Intake of lycopene-rich foods was recently associated with decreased risk for several chronic diseases. The observation that serum and tissue lycopene is more than 50% cis-lycopene, whereas tomatoes and tomato-based foods contain mainly all-trans-lycopene, has led to the hypothesis that cis-isomers of lycopene are more bioavailable. We tested this hypothesis both in vitro (study 1) and in vivo (study 2). In study 1, bile acid micelles containing crystalline lycopene were prepared. The crystalline lycopene used for these analyses was 54.4% cis-lycopene. The optically clear micelle preparation contained 75.9% cis-lycopene in repeated analyses. In study 2, mesenteric lymph duct cannulated ferrets were used to study the in vivo absorption of lycopene from Lycored(TM) (an ethyl acetate extract of tomatoes containing 5% lycopene by weight; of which 91% was all-trans lycopene). Before being anesthetized, male ferrets (n = 7) were dosed orally with 40 mg lycopene per kg body weight in soybean oil. Lymph secretions were collected, on ice, for 2 h. The residual stomach and small intestinal contents, mucosa lining, lymph secretion and serum were analyzed by HPLC. Whereas the dose, stomach and intestinal contents contained 6.2-17.5% cis-lycopene, the mesenteric lymph secretions contained significantly more, 77.4%, cis-lycopene (P < 0.01). These studies demonstrate that in ferrets, cis-isomers of lycopene are more bioavailable than trans-lycopene probably because cis-isomers are more soluble in bile acid micelles and may be preferentially incorporated into chylomicrons.