Trends in Microbiological and Antibiotic Sensitivity Patterns in Infectious Keratitis: 10-Year Experience in Mexico City
Trends in Microbiological and Antibiotic Sensitivity Patterns in Infectious Keratitis: 10-Year Experience in Mexico City
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DOI：
10.1097/ico.0000000000000428
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发表时间：
2015-07-01
期刊：
影响因子：
2.8
通讯作者：
Bautista-de Lucio, Victor M.
中科院分区：
文献类型：
--
作者：
Hernandez-Camarena, Julio C.;Graue-Hernandez, Enrique O.;Bautista-de Lucio, Victor M.
目的：报告墨西哥城一个参考中心10年期间感染性角膜炎的微生物和抗生素敏感性模式的分布和趋势。方法：在这一回顾性观察病例系列中，从2002年1月至2011年12月在墨西哥城巴伦西亚孔德眼科研究所诊断为感染性角膜炎的角膜中获取样本。结果的文化，染色，和特定的敏感性/耐药菌图为每一个microorganis.Results：共1638连续角膜刮片进行了分析。在616份样本（38%）中回收了病原体，其中细菌性角膜炎占阳性培养物的544份（88%）。观察到革兰氏阴性分离株呈非显著性增加趋势（P = 0.11）。最常见的病原体是表皮葡萄球菌，最常见的革兰阴性菌是铜绿假单胞菌。耐甲氧西林金黄色葡萄球菌（MRSA）占45%。凝固酶阴性葡萄球菌耐甲氧西林（MRCNS）率为53.7%。铜绿假单胞菌对头孢他啶的耐药性从第一阶段的15%增加到研究的最后5年的74%（P = 0.01）。万古霉素的MRSA的总体敏感性为87.5%，而99.6%的MRCNS是sensitive.Conclusions：有一个非显着增加，随着时间的推移恢复革兰氏阳性和革兰氏阴性微生物。我们观察到几乎一半的MRSA和MRCNS分离株对甲氧西林的耐药性增加。
Purpose:To report the distribution and trends in microbiological and antibiotic sensitivity patterns of infectious keratitis in a 10-year period at a reference center in Mexico City.Methods:In this retrospective observational case series, samples were obtained from corneas with a diagnosis of infectious keratitis from January 2002 to December 2011 at the Institute of Ophthalmology Conde de Valenciana in Mexico City. Results of cultures, stains, and specific sensitivity/resistance antibiograms for each microorganism were analyzed.Results:A total of 1638 consecutive corneal scrapings were analyzed. Pathogen was recovered in 616 samples (38%), with bacterial keratitis accounting for 544 of the positive cultures (88%). A nonsignificant increasing trend in gram-negative isolates (P = 0.11) was observed. The most commonly isolated pathogen was Staphylococcus epidermidis, and the most common gram-negative isolated species was Pseudomonas aeruginosa. Methicillin-resistant Staphylococcus aureus (MRSA) was present in 45% of the S. aureus isolates; meanwhile, 53.7% coagulase-negative Staphylococcus isolates were methicillin resistant (MRCNS). Pseudomonas aeruginosa resistance to ceftazidime increased from 15% in the first period to 74% for the last 5 years of the study (P = 0.01). The overall sensitivity for vancomycin of MRSA was 87.5%, whereas 99.6% of the MRCNS were sensitive.Conclusions:There was a nonsignificant increase in the recovered gram-positive and gram-negative microorganisms over time. We observed an increased resistance to methicillin in almost half of the MRSA and MRCNS isolates.