Comparison of different extractive procedures for proteins from the edible seaweeds Ulva rigida and Ulva rotundata
Comparison of different extractive procedures for proteins from the edible seaweeds Ulva rigida and Ulva rotundata
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DOI：
10.1007/bf00003945
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发表时间：
1995-12-01
影响因子：
3.3
通讯作者：
Landrein, A
中科院分区：
文献类型：
--
作者：
Fleurence, J;LeCoeur, C;Landrein, A
采用经典方法或酶法从可食用海藻石莼（Ulva rigida Agardh）和圆石莼（Ulva rotundata Bliding）中提取蛋白质。在还原条件下使用NaOH或两相系统（PEG/K2 CO 3）的方案产生最佳的蛋白质产率。由这些实验条件引起的蛋白质和多糖之间的连接的切割或限制可能解释了这些方案的效率。用SDS-PAGE分析表明，用NaOH提取的U.圆形的特征在于存在三个主要条带，其表观分子量为45600、31800和18600。从U. rigida呈现两个特异性条带，其表观分子量约为27000和12000。这些组分主要富含天冬氨酸和谷氨酸、丙氨酸、甘氨酸，并且含有很少的羟脯氨酸残基（总氨基酸含量为0.91-2.44%）。与空白方法（无酶）相比，纤维素酶的使用没有显著改善藻类蛋白质的提取。完整细胞壁中底物的弱可及性可以解释这些实验数据。使用多糖酶混合物（β-葡聚糖酶、半纤维素酶、纤维素酶）后蛋白质产率的提高部分证实了这一假设。
Proteins have been extracted from the edible seaweeds Ulva rigida Agardh and Ulva rotundata Bliding using classical or enzymatic procedures. The protocols using NaOH under reductive conditions or a two-phase system (PEG/K2CO3) produced the best protein yields. The cleavage or the limitation of the linkages between proteins and polysaccharides caused by these experimental conditions probably explains the efficiency of these protocols. In SDS PAGE, the protein fraction obtained after NaOH extraction from U. rotundata is characterised by the presence of three major bands with apparent molecular weights of 45 600, 31 800 and 18 600. The protein fraction from U. rigida presents two specific bands with apparent molecular weights of about 27 000 and 12 000. These fractions are mainly rich in aspartic and glutamic acids, alanine, glycine and contain few hydroxyproline residues (0.91-2.44% total amino acid content). The use of cellulase does not significantly improve the extraction of algal proteins in comparison with the blank procedure (without enzymes). The weak accessibility of the substrates in the intact cell wall could explain these experimental data. The improvement of protein yield after the use of the polysaccharidase mixture (beta-glucanase, hemicellulase, cellulase) partially confirms this hypothesis.