Localization and development of nerve growth factor-sensitive rat basal forebrain neurons and their afferent projections to hippocampus and neocortex
Localization and development of nerve growth factor-sensitive rat basal forebrain neurons and their afferent projections to hippocampus and neocortex
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DOI：
10.1523/jneurosci.09-09-02999.1989
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发表时间：
1989-09
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影响因子：
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通讯作者：
S. Koh;Rebekah Loy
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文献类型：
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作者：
S. Koh;Rebekah Loy
为了进一步了解神经生长因子在神经生长因子敏感的基底前脑神经元的发展及其传入连接的海马和新皮质的作用，我们使用单克隆抗体192 IgG检测和定位神经生长因子受体免疫细胞化学在发育中的大鼠大脑。神经生长因子受体免疫反应性（NGF-RI）是第一次在胚胎第13天（E13）在端脑腹外侧壁可见，并遵循尾侧到吻侧梯度在其最初的外观。NGF-RI神经元的数量和neurokinase染色经历出生前大幅增加，广泛的树突状细胞的生长和增加perikaryal大小继续在出生后的第一个3周。然而，这种增长和细胞分化，其次是在出生后第4周，后来由一个明显的减少，树突状树枝状和50%的大小收缩的胞体。最初的NGF-RI纤维生长不成熟的基底前脑神经元朝向适当的目标领域，观察早在E15。在海马终末领域的隔轴突和入侵的NB传入到皮质的层状模式的形成发生在出生后的一段时间。在海马中，NGF-RI最初是弥漫性分布的，未成熟的颗粒和锥体细胞的宽带几乎没有免疫反应性纤维，然而，随着成熟，隔轴突终末集中在狭窄的区域密切相关的细胞层。在新皮质中，早期到达的基底前脑传入神经在进入皮质之前聚集在暗免疫反应亚板下方的中间区。密集的基板和短暂存在，径向排列的纤维染色完全消失，在出生后的一周后，并逐渐取代特定的轴突和终端染色与NB传入。NGF受体的表达在subplate区的时间传入到达和随后的消失与特定的终端形成表明，NGF受体和伴随的积累的NGF在subplate可能作为一个临时目标提前到达基底前脑传入，向内传入，然后可以引导径向取向的NGF-RI纤维到适当的突触部位。
In order to understand further the role of NGF in the development of NGF-sensitive basal forebrain neurons and their afferent connections to the hippocampus and neocortex, we have used monoclonal antibody 192 IgG to detect and localize NGF receptors immunocytochemically in the developing rat brain. NGF receptor immunoreactivity (NGF-RI) is first visible at embryonic day 13 (E 13) in the ventrolateral telencephalic wall and follows a caudal-to-rostral gradient in its initial appearance. NGF-RI neuronal number and neuropil staining undergo substantial increases before birth, and extensive dendritic growth and increases in perikaryal size continue during the first 3 weeks of postnatal life. This growth and cellular differentiation, however, is followed in the fourth postnatal week and later by an apparent decrease in dendritic arborization and 50% shrinkage in the size of perikarya. Initial NGF-RI fiber outgrowth from immature basal forebrain neurons directed toward appropriate target fields is observed as early as E 15. The formation of a laminar pattern by septal axons in the hippocampal terminal fields and invasion of NB afferents into the cortex occur postnatally over a protracted time. In the hippocampus, NGF-RI is initially diffusely distributed, and wide bands of immature granule and pyramidal cells are almost devoid of immunoreactive fibers; however, with maturity, septal axon terminals become concentrated in narrow zones closely associated with the cellular layers. In the neocortex, early-arriving basal forebrain afferents accumulate in the intermediate zone underneath the darkly immunoreactive subplate before they enter The cortex. Dense subplate and transiently present, radially aligned fiber staining completely disappear in later postnatal week and are gradually replaced by specific axonal and terminal staining associated with NB afferents. The expression of NGF receptor in the subplate zone at the time afferents arrive and its subsequent disappearance with the specific terminal formation suggest that NGF receptor and concomitant accumulation of NGF in the subplate may act as a temporary target for the early-arriving basal forebrain afferents; ingrowing afferents may then be guided by radially oriented NGF-RI fibers to proper synaptic sites.