In vitro biocompatibility and oxidative stress profiles of different hydraulic calcium silicate cements.
In vitro biocompatibility and oxidative stress profiles of different hydraulic calcium silicate cements.
复制标题
不同液压钙水泥的体外生物相容性和氧化应激谱。
DOI：
10.1016/j.joen.2013.07.009
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发表时间：
2014-02
影响因子：
4.2
通讯作者：
Tay FR
中科院分区：
文献类型：
--
作者：
Eid AA;Gosier JL;Primus CM;Hammond BD;Susin LF;Pashley DH;Tay FR
MTA Plus（MTAP; Avalon Biomed Inc.，布拉登顿，佛罗里达州）是一种新的硅酸钙水泥，具有未知的细胞毒性特征。本研究的目的是检查MTA Plus对大鼠成牙本质细胞样细胞的活力、凋亡/坏死特征和氧化应激水平的影响。将MDPC-23细胞暴露于灰色和白色MTA Plus（GMTAP，WMTAP）、灰色和白色ProRoot® MTA（GMTA，WMTA; Dentsply塔尔萨牙科专业公司，塔尔萨，OK）粘固剂或其洗脱液。对细胞进行以下评价：i）使用XTT测定的细胞活力，ii）使用流式细胞术和共聚焦激光扫描显微术的凋亡/坏死，和iii）通过测量活性氧物质的氧化应激。XTT试验表明，所有测试骨水泥显示出明显的初始细胞毒性，随时间的推移而降低。到第三周结束时，GMTAP和GMTA在细胞活力方面与未处理的细胞（阴性对照）相当，而WMTAP和WMTP显著低于未处理的细胞。暴露于WMTAP和GMTAP的细胞的凋亡/坏死谱与未处理的细胞没有显著差异，而暴露于WMTA和GMTA的细胞显示出显著更少的活细胞。与未处理的细胞相比，所有实验组均表现出细胞内ROS形成的减少，尽管暴露于WMTA的细胞与未处理的细胞没有显著差异。灰色和白色版本的MTA Plus具有可忽略的体外细胞毒性风险，这些风险具有时间和稀释度依赖性。它们丰富了目前临床医生可用于根管应用的水硬性硅酸钙水门汀的范围。
MTA Plus (MTAP; Avalon Biomed Inc., Bradenton, FL) is a new calcium silicate cement with unknown cytotoxicity characteristics. The objectives of this study were to examine the effect of MTA Plus on the viability, apoptosis/necrosis profile and oxidative stress levels of rat odontoblast-like cells. MDPC-23 cells were exposed to gray and white MTA Plus (GMTAP, WMTAP), gray and white ProRoot® MTA (GMTA, WMTA; Dentsply Tulsa Dental Specialties, Tulsa, OK) cements or their eluents. The cells were evaluated for: i) cell viability using XTT assay, ii) apoptosis/necrosis using flow cytometry and confocal laser scanning microscopy, and iii) oxidative stress by measuring reactive oxygen species. XTT assay showed that all test cements exhibited marked initial cytotoxicity that decreased with time. By the end of the third week, GMTAP and GMTA were comparable to untreated cells (negative control) in terms of cell viability, while WMTAP and WMTP were significantly lower than the untreated cells. Apoptosis/necrosis profiles of cells exposed to WMTAP and GMTAP were not significantly different from untreated cells, while cells exposed to WMTA and GMTA showed significantly less viable cells. All experimental groups exhibited reduction of intracellular ROS formation compared to untreated cells, although cells exposed to WMTA was not significantly different from untreated cells. Both the gray and white versions of MTA Plus possess negligible in-vitro cytotoxic risks that are time and dilution dependent. They enrich the spectrum of hydraulic calcium silicate cements currently available to clinicians for endodontic applications.