Cricket body size is altered by systemic RNAi against insulin signaling components and epidermal growth factor receptor
Cricket body size is altered by systemic RNAi against insulin signaling components and epidermal growth factor receptor
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DOI：
10.1111/j.1440-169x.2011.01291.x
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发表时间：
2011-09-01
影响因子：
2.5
通讯作者：
Noji, Sumihare
中科院分区：
文献类型：
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作者：
Dabour, Noha;Bando, Tetsuya;Noji, Sumihare
发育生物学的一个长期存在的问题是体型是如何决定的。在果蝇中，胰岛素/胰岛素样生长因子（I/IGF）和雷帕霉素靶点（TOR）信号通路在此过程中发挥着重要作用。然而，调节昆虫身体生长的详细机制尚不清楚。因此，我们尝试利用全身若虫RNA干扰（nyRNAi）来敲低胰岛素信号成分的表达，包括胰岛素受体（InR）、胰岛素受体底物（chico）、磷酸酶和张力蛋白同源物（Pten）、雷帕霉素靶标（Tor）、RPS6-p70-蛋白激酶（S6k）、叉头盒O（FoxO）和 表皮生长因子受体（Egfr）并观察对双斑蟋蟀体型的影响。我们发现，用针对 Gryllus InR、chico、Tor、S6k 和 Egfr 的双链 RNA (dsRNA) 处理的蟋蟀表现出较小的体型，而 Gryllus FoxO nyRNAi 处理的蟋蟀则表现出比正常体型更大的体型。此外，针对Gryllus chico和Tor的RNAi显示出生长缓慢，并且针对Gryllus chico的RNAi显示出比对照蟋蟀更长的寿命。由于在 Gryllus chico(nyRNAi) 若虫和对照之间没有观察到食物摄取能力存在显着差异，我们得出结论，通过全身性 RNAi 敲低 Gryllus InR、chico、Tor、S6k、FoxO 和 Egfr 的表达，可以改变成年蟋蟀的体型大小。我们的结果表明，蟋蟀是一个很有前途的模型，可以通过 RNAi 方法研究控制身体大小和寿命的机制。
A long-standing problem of developmental biology is how body size is determined. In Drosophila melanogaster, the insulin/insulin-like growth factor (I/IGF) and target of rapamycin (TOR) signaling pathways play important roles in this process. However, the detailed mechanisms by which insect body growth is regulated are not known. Therefore, we have attempted to utilize systemic nymphal RNA interference (nyRNAi) to knockdown expression of insulin signaling components including Insulin receptor (InR), Insulin receptor substrate (chico), Phosphatase and tensin homologue (Pten), Target of rapamycin (Tor), RPS6-p70-protein kinase (S6k), Forkhead box O (FoxO) and Epidermal growth factor receptor (Egfr) and observed the effects on body size in the Gryllus bimaculatus cricket. We found that crickets treated with double-stranded RNA (dsRNA) against Gryllus InR, chico, Tor, S6k and Egfr displayed smaller body sizes, while Gryllus FoxO nyRNAi-ed crickets exhibited larger than normal body sizes. Furthermore, RNAi against Gryllus chico and Tor displayed slow growth and RNAi against Gryllus chico displayed longer lifespan than control crickets. Since no significant difference in ability of food uptake was observed between the Gryllus chico(nyRNAi) nymphs and controls, we conclude that the adult cricket body size can be altered by knockdown of expressions of Gryllus InR, chico, Tor, S6k, FoxO and Egfr by systemic RNAi. Our results suggest that the cricket is a promising model to study mechanisms underlying controls of body size and life span with RNAi methods.