SECRETION OF LIPOPROTEIN-X BY PERFUSED LIVERS OF RATS WITH CHOLESTASIS
SECRETION OF LIPOPROTEIN-X BY PERFUSED LIVERS OF RATS WITH CHOLESTASIS
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DOI：
10.1073/pnas.75.7.3459
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发表时间：
1978-01-01
影响因子：
11.1
通讯作者：
HAVEL, RJ
中科院分区：
文献类型：
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作者：
FELKER, TE;HAMILTON, RL;HAVEL, RJ
从胆道梗阻大鼠的血浆和离体肝脏的灌注液中分离出主要的胆汁淤积异常血浆脂蛋白（LP-X）。在这两种情况下，LP-X 主要由大约等摩尔部分的磷脂和未酯化的胆固醇组成。小蛋白质成分主要是富含精氨酸的载脂蛋白。通过 EM，LP-X 显示为单层脂质体（平均直径 690 埃，范围 400-1000 埃），具有磷脂双层典型的三层染色图像。用温热的乙酸铀酰（37°）对胆汁淤积的肝脏进行 48 小时的广泛块染色，可以看到肝实质内与 LP-X 无法区分的囊泡。这些三层染色囊泡主要发生在胆小管内。在附近的细胞质空泡或肝细胞之间的内陷以及迪斯间隙中也可以看到它们。在内质网或高尔基池中未观察到类似的囊泡。囊泡可在胆小管内形成并从胆小管转运至胞饮液泡内的迪斯空间。
The major abnormal plasma lipoprotein of cholestasis (LP-X) was isolated from blood plasma and from perfusates of isolated livers of rats with biliary obstruction. In both cases LP-X was composed mainly of approximately equimolar parts of phospholipids and unesterified cholesterol. The small protein component was primarily the arginine-rich apolipoprotein. By EM, LP-X appeared as a unilamellar liposome (690 .ANG. mean diameter, range 400-1000 .ANG.) with the trilaminar staining image typical of phospholipid bilayers. Extensive block staining of cholestatic livers of 48 h with warmed uranyl acetate (37.degree.) permitted the visualization of vesicles indistinguishable from LP-X within hepatic parenchyma. These trilaminar-staining vesicles occurred predominantly within bile canaliculi. They also were seen in nearby cytoplasmic vacuoles or invaginations between hepatocytes and in the space of Disse. Similar vesicles were not seen in the endoplasmic reticulum or Golgi cisternae. The vesicles may be formed within bile canaliculi and transported from the canaliculi to the space of Disse within pinocytotic vacuoles.