Bacillus anthracis pXO1 plasmid sequence conservation among closely related bacterial species
Bacillus anthracis pXO1 plasmid sequence conservation among closely related bacterial species
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DOI：
10.1128/jb.184.1.134-141.2002
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发表时间：
2002-01-01
影响因子：
3.2
通讯作者：
Kuske, CR
中科院分区：
文献类型：
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作者：
Pannucci, J;Okinaka, RT;Kuske, CR
对181.7 kb的炭疽芽孢杆菌毒力质粒pXO1进行了完整的测序和注释，预测了143个基因，但只能赋予45个可能的功能。通过杂交试验、聚合酶链式反应扩增和DNA测序来确定pXO1开放阅读框架(ORF)序列是否存在于其他细菌和更远缘的细菌属中。对18种芽孢杆菌和4种其他细菌进行了106个pXO1开放阅读框的检测。在大多数被测试的细菌中都有三个ORF保守。许多pXO1开放阅读框在关系密切的芽孢杆菌中被检测到，一些仅在炭疽杆菌分离株中检测到。蜡状芽孢杆菌D-17、蜡状芽孢杆菌43881和苏云金芽孢杆菌33679三个分离株含有的序列与所检测的pXO1ORF序列的一半以上相似。通过PCR从这些生物中扩增出的大部分DNA片段的DNA序列与pXO1的DNA序列在80%到98%之间。脉冲场凝胶电泳法显示，蜡状芽孢杆菌43881(341kb)和苏云金芽孢杆菌ATCC 33679(327kb)中都存在与6个pXO1ORF组成的探针杂交的大的潜在质粒。
The complete sequencing and annotation of the 181.7-kb Bacillus anthracis virulence plasmid pXO1 predicted 143 genes but could only assign putative functions to 45. Hybridization assays, PCR amplification, and DNA sequencing were used to determine whether pXO1 open reading frame (ORF) sequences were present in other bacilli and more distantly related bacterial genera. Eighteen Bacillus species isolates and four other bacterial species were tested for the presence of 106 pXO1 ORFs. Three ORFs were conserved in most of the bacteria tested. Many of the pXO1 ORFs were detected in closely related Bacillus species, and some were detected only in B. anthracis isolates. Three isolates, Bacillus cereus D-17, B. cereus 43881, and Bacillus thuringiensis 33679, contained sequences that were similar to more than one-half of the pXO1 ORF sequences examined. The majority of the DNA fragments that were amplified by PCR from these organisms had DNA sequences between 80 and 98% similar to that of pXO1. Pulsed-field gel electrophoresis revealed large potential plasmids present in both B. cereus 43881 (341 kb) and B. thuringiensis ATCC 33679 (327 kb) that hybridized with a DNA probe composed of six pXO1 ORFs.