Cloning and identification of the gene gob-5, which is expressed in intestinal goblet cells in mice
Cloning and identification of the gene gob-5, which is expressed in intestinal goblet cells in mice
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DOI：
10.1006/bbrc.1999.0168
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发表时间：
1999-02-16
影响因子：
3.1
通讯作者：
Hirohashi, S
中科院分区：
文献类型：
--
作者：
Komiya, T;Tanigawa, Y;Hirohashi, S
通过构建大规模原位杂交系统（Analytical Biochemistry（1997）254，23-30），我们分离了在小鼠肠杯状细胞中表达的cDNA gob-5。在小肠、结肠、胃和子宫中大量表达，在气管组织中少量表达。gob-5 cDNA的大小为3 kb。在使用我们的原位杂交方法筛选消化道组织后，我们证明gob-5在粘液细胞中表达。推导的氨基酸序列与编码牛气管上皮氯离子通道的基因相似。(C)北京：科学出版社.
By casing the large-scale in situ hybridization system (Analytical Biochemistry (1997) 254, 23-30), we isolated the cDNA gob-5 which is expressed in the intestinal goblet cells in mice. The transcript was also found to be abundantly expressed in small intestine, colon, stomach, and uterus and slightly expressed in trachea tissue. The gob-5 cDNA was shown to be 3 kb in size. After screening digestive tract tissues using our in situ hybridization method we demonstrate that gob-5 is expressed in mucous cells. The deduced amino acid sequence is similar to the gene which encodes the epithelial chloride channel in the bovine trachea. (C) 1999 Academic Press.