Effect of intramammary infusion of recombinant bovine GM-CSF and IL-8 on CMT score, somatic cell count, and milk mononuclear cell populations in Holstein cows with Staphylococcus aureus subclinical mastitis
Effect of intramammary infusion of recombinant bovine GM-CSF and IL-8 on CMT score, somatic cell count, and milk mononuclear cell populations in Holstein cows with Staphylococcus aureus subclinical mastitis
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DOI：
10.1007/s11259-017-9684-y
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发表时间：
2017-09-01
影响因子：
2.2
通讯作者：
Hayashi, Tomohito
中科院分区：
文献类型：
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作者：
Kiku, Yoshio;Ozawa, Tomomi;Hayashi, Tomohito
研究了重组牛粒细胞-巨噬细胞集落刺激因子（rbGM-CSF）和白细胞介素-8（rbIL-8）对乳区单个核细胞数、体细胞计数（SCC）和加州乳腺炎试验（CMT）评分的影响。从所选的患有自然发生的金黄色葡萄球菌亚临床乳腺炎的奶牛中，选择每头奶牛的四分之一输注rbGM-CSF（400 μ g/5 mL/乳区，n = 9）、rbIL-8（1 mg/5 mL/乳区，n = 9）和磷酸盐缓冲盐水（5 mL/乳区，n = 7）。与输注前水平相比，输注后两种细胞因子的CMT评分在第0天和第1天之间暂时增加，在第7天和第14天之间显著降低。输注rbGM-CSF后第14天的SCC有低于对照组的趋势。在输注rbGM-CSF后第1天和第2天，CD 14+细胞的百分比增加。第2天和第3天CD 4+和CD 8+细胞的百分比也增加，表明输注rbGM-CSF增强了乳腺的细胞免疫。相比之下，CD 14+细胞的百分比在输注rbIL-8后第0.25天和第1天降低。输注rbIL-8后，乳汁中CD 4 ~+和CD 8 ~+细胞的百分率在试验期间无明显变化，提示rbIL-8对乳腺T细胞功能影响不大。这些结果表明rbGM-CSF和rbIL-8通过不同的机制降低CMT评分，并可能具有作为亚临床乳腺炎治疗剂的潜力。
The effect of intramammary infusion of recombinant bovine granulocyte-macrophage colony-stimulating factor (rbGM-CSF) and interleukin-8 (rbIL-8) on mononuclear cell populations in quarters, somatic cell count (SCC) and the California Mastitis Test (CMT) score were investigated. From the selected cows with naturally occurring Staphylococcus aureus subclinical mastitis, one quarter of each cow were selected for the infusions of rbGM-CSF (400 mu g/5 mL/quarter, n = 9), rbIL-8 (1 mg/5 mL/quarter, n = 9), and phosphate-buffered saline (5 mL/quarter, n = 7). The CMT score of both cytokines post infusion temporarily increased between days 0 and 1 and significantly decreased between days 7 and 14 compared to the preinfusion level. The SCC on day 14 after infusions of rbGM-CSF tended to be lower than that of the control group. The percentage of CD14+ cells increased on days 1 and 2 post infusion of rbGM-CSF. The percentage of CD4+ and CD8+ cells also increased on days 2 and 3, suggesting that the infusion of rbGM-CSF enhanced cellular immunity in the mammary gland. In contrast, the percentage of CD14+ cells decreased on days 0.25 and 1 post infusion of rbIL-8. No significant changes in the percentages of CD4+ and CD8+ cells in milk after infusion of rbIL-8 were evident during the experimental period, which suggested that rbIL-8 had little effect on the function of T cells in the mammary gland. These results indicated that rbGM-CSF and rbIL-8 decreased the CMT score by a different mechanism and may have a potential as therapeutic agents for subclinical mastitis.