Folate-deficiency induced cell-specific changes in the distribution of lymphocytes and granulocytes in rats
Folate-deficiency induced cell-specific changes in the distribution of lymphocytes and granulocytes in rats
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DOI：
10.1007/s12199-012-0286-6
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发表时间：
2013-01-01
影响因子：
4.7
通讯作者：
Imaizumi, Kazuhiko
中科院分区：
文献类型：
--
作者：
Abe, Ikumi;Shirato, Ken;Imaizumi, Kazuhiko
目的叶酸(维生素B-9)通过调节DNA和RNA的合成和稳定在细胞的生长和增殖中起关键作用，其缺乏会导致淋巴细胞减少和粒细胞减少。然而，叶酸缺乏究竟如何影响各种白细胞亚群的分布，包括少量嗜碱性粒细胞，以及这些影响的细胞特异性仍不清楚。因此，我们研究了叶酸缺乏饮食对大鼠外周血淋巴细胞亚群[T淋巴细胞、B淋巴细胞和自然杀伤细胞]和粒细胞亚群(中性粒细胞、嗜酸性粒细胞和嗜碱性粒细胞)循环数量的影响。方法将大鼠分为两组，一组为叶酸缺乏组(FAD组)，另一组为对照组(CON组)。结果FAD组大鼠血浆叶酸水平显著低于CON组，血浆同型半胱氨酸水平显著高于CON组，血浆同型半胱氨酸水平是叶酸缺乏的预测指标。FAD组T淋巴细胞、B淋巴细胞和NK细胞数量分别比CON组低0.73倍、0.49倍和0.70倍，表明B淋巴细胞对叶酸缺乏的敏感性高于其他淋巴细胞亚群。正如预期的那样，FAD组的中性粒细胞和嗜酸性粒细胞数量显著低于CON组。结论叶酸缺乏可引起细胞特异性淋巴细胞减少和粒细胞减少。
Objective Folate (vitamin B-9) plays key roles in cell growth and proliferation through regulating the synthesis and stabilization of DNA and RNA, and its deficiency leads to lymphocytopenia and granulocytopenia. However, precisely how folate deficiency affects the distribution of a variety of white blood cell subsets, including the minor population of basophils, and the cell specificity of the effects remain unclear. Therefore, we examined the effects of a folate-deficient diet on the circulating number of lymphocyte subsets [T-lymphocytes, B-lymphocytes, and natural killer (NK) cells] and granulocyte subsets (neutrophils, eosinophils, and basophils) in rats.Methods Rats were divided into two groups, with one receiving the folate-deficient diet (FAD group) and the other a control diet (CON group). All rats were pair-fed for 8 weeks.Results Plasma folate level was dramatically lower in the FAD group than in the CON group, and the level of homocysteine in the plasma, a predictor of folate deficiency was significantly higher in the FAD group than in the CON group. The number of T-lymphocytes, B-lymphocytes, and NK cells was significantly lower in the FAD group than in the CON group by 0.73-, 0.49-, and 0.70-fold, respectively, indicating that B-lymphocytes are more sensitive to folate deficiency than the other lymphocyte subsets. As expected, the number of neutrophils and eosinophils was significantly lower in the FAD group than in the CON group. However, the number of basophils, the least common type of granulocyte, showed transiently an increasing tendency in the FAD group as compared with the CON group.Conclusion These results suggest that folate deficiency induces lymphocytopenia and granulocytopenia in a cell-specific manner.