Cytotoxicity of endodontic materials
Cytotoxicity of endodontic materials
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DOI：
10.1016/s0099-2399(98)80084-8
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发表时间：
1998-02-01
影响因子：
4.2
通讯作者：
Shawley, AL
中科院分区：
文献类型：
--
作者：
Osorio, RM;Hefti, A;Shawley, AL
采用人牙龈成纤维细胞和L细胞的体外细胞培养模型，检测了目前常用的根管封闭剂Endomet、CRCs、AH26和根管充填材料银汞合金、镓GF2、Ketac Silver、三氧化矿物聚合体和Super-EBA的细胞毒性。用四甲基偶氮唑盐比色法测定线粒体酶活性，用变异系数法测定细胞数，评价细胞毒作用。采用贴片培养和L-929成纤维细胞。All-Bond-2也进行了评估，统计分析结果显示，CRCs是细胞毒性最小的封闭剂，其次是Endomet和AH26。在根尖充填材料中，MTA无细胞毒性，镓GF2细胞毒性较小，Ketac Silver、Super-EBA和银汞合金具有较高的细胞毒性。所有Bond-2均表现出高度的细胞毒性，其中CRCs是最好的根管封闭剂，MTA是最好的根管充填材料。结果也是有利的，镓GF2作为回填材料。
An in vitro cell culture model of human gingival fibroblasts and L-929 cells was used to measure the cytotoxicity of currently used root canal sealers Endomet, CRCS, and AH26 and root-end filling materials Amalgam, Gallium GF2, Ketac Silver, mineral trioxide aggregate (MTA), and Super-EBA. Cytotoxic effects were assessed using the MTT assay for mitochondrial enzyme activity and the CV assay for cell numbers. Using inserts culture and L-929 fibroblasts. All-Bond-2 was also evaluated, The statistical analysis of results showed that CRCS was the least cytotoxic sealer followed by Endomet and AH26. Among root-end filling materials, MTA was not cytotoxic; Gallium GF2 displayed little cytotoxicity; and Ketac Silver, Super-EBA, and Amalgam showed higher levels of cytotoxicity. All Bond-2 also displayed a high degree of cytotoxicity, CRCS was the best root canal sealer and MTA the best root-end filling material. The outcome was favorable also for Gallium GF2 as a retrofilling material.