Characteristics of some high molecular weight constituents with bacterial aggregating activity from whole saliva and dental plaque.
Characteristics of some high molecular weight constituents with bacterial aggregating activity from whole saliva and dental plaque.
复制标题
全唾液和牙菌斑中一些具有细菌聚集活性的高分子量成分的特征。
作者：
D. I. Hay;R. Gibbons;D. Spinell
本研究的目的是调查人类全唾液中存在的导致某些口腔细菌聚集的因子的性质。引起聚集的因素似乎可能在牙菌斑形成中起作用，因为它可以促进生物体粘附到牙齿上，并有助于牙菌斑中生物体的相互粘附。人全唾液的琼脂糖柱层析得到含有聚集活性的空体积峰。对该材料的研究表明，它含有33%的蛋白质、19%的蒽酮阳性碳水化合物、2.9%的N-乙酰神经氨酸和大量的己糖胺。超电泳分析、琼脂糖柱层析、氨基酸分析、等电聚焦和十六烷基三甲基溴化铵分级分离的数据表明，活性物质是以无规卷曲构型存在于溶液中的高分子量糖蛋白。进一步发现，活性材料被选择性地吸附到羟基磷灰石表面上，并且还可以从牙菌斑中分离出具有与上述性质类似的性质的组分。因此，在整个唾液中似乎存在一种高分子量糖蛋白，其选择性地吸附在磷灰石表面上并引起某些口腔生物体的聚集。似乎该组分在某些口腔生物体对牙齿表面的初始选择性粘附中起重要作用，以及参与生物体在形成菌斑中的相互粘附。
The aim of this study was to investigate the nature of a factor present in human whole saliva which causes the aggregation of certain oral bacteria. It seemed possible that the factor causing aggregation could play a part in plaque formation in that it could promote adhesion of organisms to the teeth and contribute to the mutual adhesion of the organisms in the plaque. Agarose column chromatography of human whole saliva gave a void volume peak which contained the aggregating activity. Investigation of this material showed it to contain 33% protein, 19% anthrone positive carbohydrate, 2.9% N-acetyl neuraminic acid and substantial amounts of hexosamine. Data from ultracentrifugal analysis, agarose column chromatography, amino acid analysis, isoelectric focusing and cetyl trimethylammonium bromide fractionation indicated that the active material was a high molecular weight glycoprotein which exists in solution in a random coil configuration. It was further found that the active material was selectively adsorbed on to the hydroxy-apatite surface and also that a component with properties similar to those described above could be isolated from dental plaque. It seems, therefore, that there is, in whole saliva, a high molecular weight glycoprotein, which selectively adsorbs on to the apatite surface and which causes aggregation of certain oral organisms. It seems possible that this component plays a significant role in the initial selective adhesion of certain oral organisms to the tooth surface, as well as being involved in the mutual adhesion of the organisms in the developing plaque.