Effect of Glucose on the Fruiting Body Formation and Adenosine 3′,5′-Cyclic Monophosphate Levels in Coprinus macrorhizus
Effect of Glucose on the Fruiting Body Formation and Adenosine 3′,5′-Cyclic Monophosphate Levels in Coprinus macrorhizus
复制标题
葡萄糖对鸡腿菇子实体形成及3′,5′-环磷酸腺苷水平的影响
DOI：
10.1128/jb.120.1.96-100.1974
复制
发表时间：
1974
影响因子：
3.2
通讯作者：
T. Ishikawa
中科院分区：
文献类型：
--
作者：
I. Uno;T. Ishikawa
在高糖培养基中生长抑制了单核体和双核体的子实体形成。在高糖培养基中，子实体形成过程中没有环磷酸腺苷（cAMP）积累的特征性爆发。酶活性测定表明，在高葡萄糖培养基中生长的菌丝体含有相对较低的量的腺苷酸环化酶和cAMP-磷酸二酯酶比菌丝体生长在低葡萄糖培养基。诱导型d-丝氨酸脱氨酶和天冬氨酸酶的合成在高糖培养基中受到抑制。突变体（gluR），其中的葡萄糖阻遏子实体形成的影响是通过选择在高葡萄糖培养基中分离。该突变导致cAMP水平不再受葡萄糖的影响，并影响合成诱导型d-丝氨酸脱氨酶和天冬氨酸酶的能力。gluR突变体在双核体中为部分显性。表明cAMP在诱导子实体形成和控制诱导酶合成中起重要作用。巨根
The formation of fruiting bodies in the monokaryotic fisc strain and a dikaryon of Coprinus macrorhizus was inhibited by growth in high-glucose media. In high-glucose media the characteristic burst of adenosine 3′,5′-cyclic monophosphate (cAMP) accumulation during fruiting-body formation was absent. Enzymatic activity assays revealed that mycelia grown in high-glucose media contained relatively lower amounts of adenylate cyclase and cAMP-phosphodiesterase than mycelia grown in low-glucose media. The synthesis of inducible d-serine deaminase and tryptophanase was repressed in high-glucose media. A mutant (gluR) in which the glucose repression of fruiting-body formation is affected was isolated by selection in high-glucose media. The mutation caused the cAMP levels to be no longer affected by glucose and affected ability to synthesize the inducible d-serine deaminase and tryptophanase. The gluR mutant was partially dominant in dikaryons. It is suggested that cAMP may play important roles in inducing fruiting bodies and in controlling inducible enzyme synthesis in C. macrorhizus.