ANTIGENIC CONSERVATION AND DIVERGENCE BETWEEN THE VIRAL-SPECIFIC PROTEINS OF POLIOVIRUS TYPE-1 AND VARIOUS PICORNAVIRUSES
ANTIGENIC CONSERVATION AND DIVERGENCE BETWEEN THE VIRAL-SPECIFIC PROTEINS OF POLIOVIRUS TYPE-1 AND VARIOUS PICORNAVIRUSES
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DOI：
10.1016/0042-6822(85)90441-6
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发表时间：
1985-01-01
期刊：
影响因子：
3.7
通讯作者：
WIMMER, E
中科院分区：
文献类型：
--
作者：
EMINI, EA;SCHLEIF, WA;WIMMER, E
用脊髓灰质炎病毒1型特异性结构蛋白和非结构蛋白的抗血清对各种微小核糖核酸病毒感染的[人宫颈癌HeLa]细胞裂解物进行了免疫沉淀分析。结果显示了不同的抗原保守性和差异性。VP3和2C多肽在绝大多数病毒中具有很强的抗原性保守性。考虑到其他病毒蛋白表现出的差异程度，这种保守尤其值得注意，可能是由于与宿主细胞相互作用所施加的环境压力。这一结果有助于分析所测试病毒的进化关系。这项分析表明，脊髓灰质炎病毒组的蛋白与柯萨奇病毒A21之间存在特别强的抗原性关系，而与柯萨奇病毒B1和B3之间的抗原性关系较弱，但意义重大。
Immunoprecipitation analyses of various picornavirus-infected [human cervical carcinoma HeLA] cell lysates were performed using antisera to poliovirus type 1-specific structural and nonstructural proteins. The results showed differing patterns of antigenic conservation and divergence. The VP3 and 2C polypeptides were strongly antigenically conserved among the large majority of these viruses. This conservation was especially notable given the degree of divergence exhibited by the other viral proteins and may be due to environmental pressure exerted by interaction with the host cell. The results allowed for an analysis of the evolutionary relationship of the tested viruses. This analysis showed a particularly strong antigenic relationship betwen the proteins of the poliovirus group and coxsackievirus A21 as well as a weaker, but significant, relationship with coxsackieviruses B1 and B3.