The supramamillo-hippocampal and supramammillo-septal glutamatergic/aspartatergic projections in the rat:: A combined [3H]D-aspartate autoradiographic and immunohistochemical study
The supramamillo-hippocampal and supramammillo-septal glutamatergic/aspartatergic projections in the rat:: A combined [3H]D-aspartate autoradiographic and immunohistochemical study
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DOI：
10.1016/s0306-4522(00)00127-5
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发表时间：
2000-01-01
期刊：
影响因子：
3.3
通讯作者：
Leranth, C
中科院分区：
文献类型：
--
作者：
Kiss, J;Csáki, A;Leranth, C
已经确定，乳头体上核通过其与海马的直接和间接（经由隔复合体）连接在海马θ节律产生/调节中起关键作用。以往的形态学和电生理学研究表明，乳头上海马和乳头上隔传出都含有兴奋性递质。为了测试这一假设的有效性，发射器特定的逆行示踪剂实验进行。将[H-3] D-天冬氨酸注射到海马的不同位置（齿状回的颗粒层和颗粒上层以及阿蒙环的CA 2和CA 3a区域）和隔膜复合体（内侧隔膜以及内侧和外侧隔膜之间的区域），这些位置是乳头体上投射的已知靶点。从整个长度的后下丘脑，包括乳头体上区制备的连续振动切片，进行钙视网膜蛋白，酪氨酸羟化酶，或钙结合蛋白免疫染色，并进一步处理放射自显影。放射性标记，放射性标记加钙网膜含，钙网膜免疫反应神经元绘制在6个不同的oro-caudal水平的乳头体上区。结果表明，在海马和隔注射示踪剂后，大多数逆行放射性标记的细胞（钙网蛋白能/钙网蛋白能）对钙网蛋白呈免疫反应性。然而，非放射性标记的calretinin含有神经元和放射性标记的calretininin-immunogonnegative细胞也被看到，虽然在一个低得多的density.These观察清楚地表明存在的海马和隔复合体的突触/突触投射。可以假设，该递质可能在海马θ节律产生/调节中发挥作用。(C)2000年IBRO，Elsevier Science Ltd.出版。
It is well established that the supramammillary nucleus plays a critical role in hippocampal theta rhythm generation/regulation by its direct and indirect (via the septal complex) connections to the hippocampus. Previous morphological and electrophysiological studies indicate that both the supramammillo-hippocampal and supramammillo-septal efferents contain excitatory transmitter. To test the validity of this assumption, transmitter specific retrograde tracer experiments were performed. [H-3]D-aspartate was injected into different locations of the hippocampus (granular and supragranular layers of the dentate gyrus and CA2 and CA3a areas of the Ammon's hem) and septal complex (medial septum and the area between the medial and lateral septum) that are known targets of the supramammillary projection. Consecutive vibratome sections prepared from the entire length of the posterior hypothalamus, including the supramammillary area, were immunostained for calretinin, tyrosine hydroxylase, or calbindin, and further processed for autoradiography. Radiolabeled, radiolabeled plus calretinin-containing, and calretinin-immunoreactive neurons were plotted at six different oro-caudal levels of the supramammillary area. The results demonstrated that following both hippocampal and septal injection of the tracer, the majority of the retrogradely radiolabeled (glutamatergic/aspartatergic) cells are immunoreactive for calretinin. However, non-radiolabeled calretinin-containing neurons and radiolabeled calretinin-immunonegative cells were also seen, albeit at a much lower density.These observations clearly indicate the presence of glutamatergic/aspartatergic projections to both the hippocampus and septal complex. It may be assumed that this transmitter could play a role in hippocampal theta rhythm generation/regulation. (C) 2000 IBRO, Published by Elsevier Science Ltd.