EFFECTS OF CALCITONIN ON OSTEOCLASTS IN-VIVO - ULTRASTRUCTURAL AND HISTOCHEMICAL STUDY
EFFECTS OF CALCITONIN ON OSTEOCLASTS IN-VIVO - ULTRASTRUCTURAL AND HISTOCHEMICAL STUDY
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DOI：
10.1007/bf00307190
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发表时间：
1973-01-01
期刊：
影响因子：
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通讯作者：
LUCHT, U
中科院分区：
文献类型：
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作者：
LUCHT, U
用电镜观察了猪降钙素对幼龄大鼠胫骨后端破骨细胞的影响。注射激素后1 1/2、4、8或12小时处死动物。在光镜检查的调查切片中，破骨细胞比对照动物小。在超微结构水平上，破骨细胞表现出以下变化：1)典型的皱褶边界缺失。2)细胞与骨间的胞外间隙不存在酸性磷酸酶。3)大液泡数量减少，细胞质内不存在局部液泡积聚。4)空泡中不含骨晶体。5)细胞器泡增多。这些液泡大部分被鉴定为自溶酶体，因为它们含有酸性磷酸酶，封闭的细胞器被部分消化。上述变化在研究的所有时间间隔都存在。研究结果表明，降钙素可减少或抑制破骨细胞的骨吸收。破骨细胞功能的下降可能导致激素的低钙效应。自噬酶体数量增加是自噬作用增强的证据。讨论了破骨细胞中自溶酶体的可能起源。
Osteoclasts from the tibial metaphyses of young rats treated with porcine calcitonin were studied by electron microscopy. The animals were sacrificed 1 1/2, 4, 8 or 12 hours after injection of the hormone. In survey sections examined by light microscopy the osteoclasts appeared smaller than in control animals. At the ultrastructural level the osteoclasts showed the following alterations: 1) The typical ruffled border was absent. 2) Acid phosphatase was not present in the extracellular space between cell and bone. 3) The number of large vacuoles was decreased and there was no local accumulation of vacuoles in the cytoplasm. 4) The vacuoles did not contain bone crystals. 5) Vacuoles with cell organelles were increased in number. The majority of these vacuoles were identified as autolysosomes because they contained acid phosphatase and the enclosed cell organelles were partially digested. The above changes were present at all time intervals studied.The findings suggest that calcitonin decreases or inhibits bone resorption by osteoclasts. A decreased function of the osteoclasts may contribute to the hypocalcemic effect of the hormone. The increased number of autolysosomes is evidence of an enhanced autophagocytosis. Possible origins of the autolysosomes in osteoclasts are discussed.