Accumulation of quantitative trait loci conferring broad-spectrum clubroot resistance in Brassica oleracea
Accumulation of quantitative trait loci conferring broad-spectrum clubroot resistance in Brassica oleracea
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DOI：
10.1007/s11032-013-9918-9
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发表时间：
2013-12-01
影响因子：
3.1
通讯作者：
Okazaki, Keiichi
中科院分区：
文献类型：
--
作者：
Tomita, Hiroya;Shimizu, Motoki;Okazaki, Keiichi
根肿病是世界上危害甘蓝最严重的病害之一。为了发展标记辅助选择（MAS），可以帮助将持久根肿病抗性（CR）纳入品种，以前的遗传分析已经确定了几个CR数量性状位点（CR-QTL）。然而，每个CR基因座对各种分离株的独立和累积效应很少被测试。在此之前，我们在青花菜双单倍体（DH）系×甘蓝DH系B的F2植株中鉴定了1个主效和4个次效CR-QTL。甘蓝属植物在本研究中，为了阐明它们对控制涉及各种分离物的疾病的有效性，在具有CR基因的各种组合的基因型中进行接种测试，所述CR基因是使用与每个CR-QTL密切相关的DNA标记选择的。在探索总体疾病发病率时，很明显，位于PbBo（Anju）1基因座中的主要CR基因的单一参与或次要CR-QTL中CR基因的积累不足以赋予足够的抗性。在QTL PbBo（Anju）1位点上的一个主效CR基因加上2 ~ 3个次效CR基因可获得中等抗性。包括PbBo（Anju）1在内的5个QTL上的CR基因全部积累的基因型表现出最高的抗性，对6个菌株表现出广泛的抗性。利用分子标记辅助选择技术积累多个CR基因是在B进行CR育种的必要条件。甘蓝。我们开发的DNA标记可以有效地用于选择获得抗性所需的位点，这些标记的使用将是B的CR育种的有力工具。甘蓝属植物
Throughout the world, clubroot disease is one of the most damaging diseases affecting Brassica oleracea. To develop marker-assisted selection (MAS) that could assist the incorporation of durable clubroot resistance (CR) into cultivars, previous genetic analyses have identified several CR quantitative trait loci (CR-QTL). However, the independent and cumulative effects of each CR locus against various isolates have rarely been tested. Previously, we identified one major CR-QTL and four minor CR-QTL in the F2 plants from broccoli doubled haploid (DH) line x cabbage DH line of B. oleracea. In the present study, to clarify their effectiveness for controlling disease involving various isolates, inoculation testing was conducted in genotypes with various combinations of the CR genes, which were selected using the DNA markers closely associated with each CR-QTL. In exploring the overall disease incidence, it was apparent that a single involvement of the major CR gene located in the PbBo(Anju)1 locus, or accumulation of CR genes in the minor CR-QTL, is not enough to confer sufficient resistance. One major CR gene in the QTL PbBo(Anju)1 locus plus two to three minor CR genes conferred moderate resistance. The genotype in which all of the CR genes locating in the five QTL including PbBo(Anju)1 were accumulated showed the highest resistance, and it was broadly resistant against six isolates. Accumulation of several CR genes by MAS is necessary to conduct CR breeding in B. oleracea. Our developed DNA markers can be used efficiently to make selections of required loci for the acquisition of resistance, and use of these markers will be a powerful tool for CR breeding in B. oleracea.