MicroRNAs involving in cold, wounding and salt stresses in Triticum aestivum L.
MicroRNAs involving in cold, wounding and salt stresses in Triticum aestivum L.
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DOI：
10.1016/j.plaphy.2014.03.020
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发表时间：
2014-07-01
影响因子：
6.5
通讯作者：
Kang, Zhen-sheng
中科院分区：
文献类型：
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作者：
Wang, Bing;Sun, Yan-fei;Kang, Zhen-sheng
microRNA（miRNAs）在转录后调控中起着重要作用，是多种逆境胁迫的重要内源性调节因子。低温、伤害和高盐是影响作物生长发育的三种常见环境胁迫刺激。在这项研究中，我们在小麦中鉴定了31个已知的miRNAs和3个新的miRNAs。此外，使用RT-qPCR数据，从已知的miRNAs中调查了19种胁迫调节的miRNAs，其中三种胁迫的影响。其中16个、12个和8个miRNAs分别在低温、创伤和高盐处理下受到调控。其中4个miRNAs通过北方杂交检测到对冷胁迫有高度响应，另外6个miRNAs与伤害调控有关，3个与高盐胁迫有关。同时，miR 159、miR 393和miR 398对多种应激刺激有反应。此外，2个新的miRNAs受到冷胁迫的调控。而miR 159、miR 398和miR 6001则可能参与了植物对逆境胁迫的反应。综上所述，本研究的结果表明，小麦miRNAs可能在响应非生物胁迫中发挥重要作用。(C)2014年Elsevier Masson SAS。All rights reserved.
MicroRNAs (miRNAs) play critical roles in post-transcriptional regulation and act as important endogenous regulators to various stresses. Cold, wounding and high-salinity are three common environmental stress stimuli influencing crops growth and development. In this study, we identified 31 known miRNAs and 3 novel miRNAs in wheat. Moreover, 19 stress-regulated miRNAs using RT-qPCR data in which the effects of three stresses were surveyed from the known miRNAs. Among them, 16, 12 and 8 miRNAs were regulated under cold, wounding and high-salinity treatments, respectively. Of which 4 miRNAs were highly responsive to cold stress in wheat by northern blot, and 6 wounding-regulated and 3 high-salinity-regulated miRNAs were detected. Meanwhile, miR159, miR393 and miR398 were responsive to multiple stress stimuli. Besides, 2 novel miRNAs were regulated by cold stress. While, the analyses of targets suggested miR159, miR398 and miR6001 could responses to stress conditions in regulation pathways. Taken together, the results of this study suggest that wheat miRNAs may play important roles in response to abiotic stress. (C) 2014 Elsevier Masson SAS. All rights reserved.