Stock identification and spawning depth of oval squid <I>Sepioteuthis</I> spp. in Tanega-shima Island inferred by DNA markers
Stock identification and spawning depth of oval squid <I>Sepioteuthis</I> spp. in Tanega-shima Island inferred by DNA markers
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椭圆形鱿鱼<I>Sepioteuthis</I> spp.的种群鉴定和产卵深度。
DOI：
10.11233/aquaculturesci.63.39
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发表时间：
2015
影响因子：
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通讯作者：
笘野 哲史・上田 幸男・ 笠岡 祝安・海野 徹也
中科院分区：
文献类型：
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作者：
Gustavo Sanchez;Satoshi Tomano;Tetsuya Umino;Toshie Wakabayashi and Mitsuo Sakai;笘野 哲史・上田 幸男・ 笠岡 祝安・海野 徹也
椭圆形乌贼Sepioteuthis spp.用10个微卫星座位和线粒体DNA中细胞色素氧化酶亚基I(COI)的部分核苷酸序列对谷儿岛岛近岸海域进行了检测。此外，我们还通过对20-50m深的人工鱼礁上采集的卵进行鉴定，提出了产卵深度。采自谷儿岛的成虫标本，经鉴定为Akaika Sepioteuthis sp.1根据表型性状和德岛水域(简称Shiroika Sepioteuthis sp.)2)在微卫星等位基因频率和mtDNA序列上存在遗传差异，同工酶分析支持生殖隔离的存在。在3个40~50m的人工鱼礁上采集的卵和胚胎被鉴定为Sepioteuthis sp.预置结果表明，Sepioteuthis sp.生活在Tanega-shima岛的鱼利用40-50米的水深作为产卵区。在本研究中使用的部分COI序列可以推断该种之间的系统发育关系。产生于印度-太平洋地区，并检测到Sepioteuthis sp.的共存。1和sp.越南和印度尼西亚有2个。
Stock identification of oval squid Sepioteuthis spp. in the coastal waters of Tanega-shima Island were examined using ten polymorphic microsatellite loci and partial nucleotide sequences of cytochrome oxidase subunit I (COI) in the mtDNA. Also, we suggested spawning depth through eggs identification collected from artificial reefs from 20-50 m in depth. Adult specimens collected from Tanega-shima Island, identified as Akaika Sepioteuthis sp. 1 based on phenotypic characters and Tokushima waters (referred as Shiroika Sepioteuthis sp. 2) were genetically differentiated in both microsatellite allele frequencies and mtDNA sequences, which supported presence of reproductive isolation inferred by isozyme analysis. Eggs and embryos at three artificial reefs from 40-50 m in depth were identified as Sepioteuthis sp. 1, except for 20 m. Preset results indicate that Sepioteuthis sp. 1 living in Tanega-shima Island utilize 40-50 m in depth as a spawning zone. The partial COI sequences used in the present study allowed inferring phylogenetic relationship among Sepioteuthis spp. generated from Indo-Pacific region and detecting co-existence of Sepioteuthis sp. 1 and sp. 2 in Vietnam and Indonesia.