DIFFERENTIATION OF MOUSE EMBRYONIC STEM-CELLS IN-VITRO .3. MORPHOLOGICAL EVALUATION OF TISSUES DEVELOPED AFTER IMPLANTATION OF DIFFERENTIATED MOUSE EMBRYOID BODIES
DIFFERENTIATION OF MOUSE EMBRYONIC STEM-CELLS IN-VITRO .3. MORPHOLOGICAL EVALUATION OF TISSUES DEVELOPED AFTER IMPLANTATION OF DIFFERENTIATED MOUSE EMBRYOID BODIES
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DOI：
10.1002/aja.1001970306
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发表时间：
1993-07-01
影响因子：
2.5
通讯作者：
KOSCO, M
中科院分区：
文献类型：
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作者：
CHEN, U;KOSCO, M
小鼠胚胎干细胞（ES）在液体培养系统中分化。 2-3周后，复杂的囊性胚状体发育。这些身体由几种结构组成，这些结构被确定为心肌和卵黄囊血岛以及含有混合造血干细胞群的杯形隔室。当这些囊性胚状体被植入成年小鼠皮下或肾囊下时，它们发育成各种组织。这些包括骨骼、血管、心肌、神经和带毛囊的皮肤。此外，还衍生出高度分化、复杂的组织，如具有粘液腺或唾液腺组织的肠上皮。来自这些体外发育的胚状体的ES组织在植入后2至3周内迅速发育。这与源自胚胎癌细胞和/或直接植入未分化胚胎干细胞的畸胎癌的最短 6 周形成鲜明对比。此外，我们发现不同的植入部位发育出不同类型的组织。数据表明局部环境和/或生长因子对 ES 组织发育有影响。该系统提供了一种可能的方法来纯化和鉴定产生特定组织的干细胞，并研究调节这些干细胞定向的因素。 (C) 1993 Wiley-Liss, Inc.
Mouse embryonic stem cells (ES) were allowed to differentiate in a liquid culture system. After 2-3 weeks, complex cystic embryoid bodies developed. These bodies were composed of several structures identified as cardiac muscle and yolk sac blood islands as well as cup-shape compartments containing a mixed population of hematopoietic stem cells. When these cystic embryoid bodies were implanted into adult mice, either subcutaneously or under the kidney capsule, they developed into various tissues. These included bone, blood vessels, cardiac muscle, nerves, and skin with hair follicles. In addition, highly differentiated, complicated tissues resembling intestinal epithelium with mucus glands or salivary glandular tissue were derived. The ES tissues from these in vitro developed embryoid bodies developed quickly within 2 to 3 weeks of implantation. This is in contrast to a minimal of 6 weeks for teratocarcinomas derived from embryonic carcinoma cells and/or the direct implantation of undifferentiated embryonic stem cells. Moreover, we found that there are different types of tissue developed upon different sites of implantation. The data suggest a local environment and/or growth factors are influential for ES tissue development. This system provides a possible means to purify and identify stem cells that give rise to specific tissues, and to study the factors regulating the commitment of these stem cells. (C) 1993 Wiley-Liss, Inc.