Mapping out Min protein patterns in fully confined fluidic chamber
Mapping out Min protein patterns in fully confined fluidic chamber
复制标题
DOI：
10.7554/elife.19271
复制
发表时间：
2016-11-25
期刊：
影响因子：
7.7
通讯作者：
Dekker, Cees
中科院分区：
文献类型：
--
作者：
Caspi, Yaron;Dekker, Cees
细菌Min蛋白系统为研究生物学中的反应扩散过程提供了一个主要的模型系统。在这里，我们提出了第一个在体外研究的最小系统在完全封闭的三维腔室，光刻定义，脂质双层涂层和隔离通过压力阀。我们确定了三个典型的动力学行为，发生依赖于腔室的几何参数：极到极振荡，螺旋旋转，行波。我们建立的几何选择规则，并表明，令人惊讶的是，最小蛋白质螺旋旋转治理的几何相图的较大部分。限制以及升高的温度降低了最小模式的特征波长，尽管即使对于具有细菌水平粘度的封闭腔室，模式也保持类似于体内5倍的波长。我们的研究结果提供了一个必不可少的实验基础建模的细胞内最小梯度在细菌细胞分裂，以及更一般地说，为理解反应扩散系统中的图案形成。
The bacterial Min protein system provides a major model system for studying reaction-diffusion processes in biology. Here we present the first in vitro study of the Min system in fully confined three-dimensional chambers that are lithography-defined, lipid-bilayer coated and isolated through pressure valves. We identify three typical dynamical behaviors that occur dependent on the geometrical chamber parameters: pole-to-pole oscillations, spiral rotations, and traveling waves. We establish the geometrical selection rules and show that, surprisingly, Min-protein spiral rotations govern the larger part of the geometrical phase diagram. Confinement as well as an elevated temperature reduce the characteristic wavelength of the Min patterns, although even for confined chambers with a bacterial-level viscosity, the patterns retain a similar to 5 times larger wavelength than in vivo. Our results provide an essential experimental base for modeling of intracellular Min gradients in bacterial cell division as well as, more generally, for understanding pattern formation in reaction-diffusion systems.