Enzymatic radioiodination of gonadotropins.
Enzymatic radioiodination of gonadotropins.
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促性腺激素的酶促放射性碘化。
DOI：
10.1210/jcem-34-1-23
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发表时间：
1972
期刊：
影响因子：
--
通讯作者：
M. Lipsett
中科院分区：
文献类型：
--
作者：
Yukitaka Miyachi;J. Vaitukaitis;Eberhard Nieschlag;M. Lipsett
本文描述了一种用乳酸过氧化物酶、过氧化氢和Na 125I组成的体系对HCG、促卵泡激素（HFSH）和促黄体生成素（HLH）进行酶促放射碘化的方法。这些激素对碘的敏感性不同。HCG很容易碘化，但HFSH和HLH需要稍高浓度的酶和过氧化氢。这些激素被标记为符合放射免疫测定的特定活性。这些制剂的生物活性保持不变（HCG 108%, HFSH >62%, HLH 99%）。这些示踪剂的聚丙烯酰胺凝胶电泳（PAGE）与它们的放射性纯度一致。乳过氧化物酶催化碘化是一种温和、简单、快速的方法，所制备的放射性碘化制剂适合于放射免疫测定。
A method is described for the enzymatic radioiodination of HCG, human follicle stimulating hormone (HFSH) and human luteinizing hormone (HLH) by a system consisting of lactoperoxidase, hydrogen peroxide and Na 125I. The hormones differed in their susceptibility to iodination. HCG was easily iodinated but HFSH and HLH required slightly higher concentrations of the enzyme and hydrogen peroxide. These hormones were labeled at specific activities satisfactory for radioimmunoassay. Biologic activities of these preparations were retained (HCG 108%, HFSH >62% and HLH 99%). Polyacrylamide gel electrophoresis (PAGE) of these tracers was consistent with their radiopurity. This lactoperoxidase catalyzed iodination is a gentle, simple and rapid method, and the radioiodinated preparations are suitable for radioimmunoassay.