Identification of diverse BoLA DQA3 genes consistent with non-allelic sequences.
Identification of diverse BoLA DQA3 genes consistent with non-allelic sequences.
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DOI：
10.1046/j.1365-2052.1998.00296.x
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发表时间：
1998-04
期刊：
影响因子：
2.4
通讯作者：
K. Ballingall;B. Marasa;Anthony Luyai;D. McKeever
中科院分区：
文献类型：
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作者：
K. Ballingall;B. Marasa;Anthony Luyai;D. McKeever
牛主要组织相容性复合体(MHC)DQA基因内的遗传多样性具有多个多态位点的特征，这些多态位点的数量在不同单倍型之间可能不同。先前对来自基因组BOLA DQA3基因的第二外显子序列的分析确定了两个不同的家族，DQA3*01和DQA3*02。在这份报告中，我们描述了代表这些家族的三个转录的BOLA DQA3基因的整个编码区的核苷酸和预测的氨基酸序列。这些数据进一步证明了BOLA DQA3基因座不同于BOLA DQA1和BOLA DQA2。此外，这两个家族的DQA3基因的氨基酸序列在254个氨基酸中有35个不同。在DQA3*01和DQA3*02基因中，DQA基因跨膜区和胞内区推测的位点特异性氨基酸序列基序不同。系统发育分析显示，遗传距离比同源MHC等位基因家族之间的遗传距离要大得多。这些数据与一个非常不同的DQA3基因家族或另外一个BOLA DQA4基因座的等位基因是一致的。
Genetic diversity within the DQA genes of the major histocompatibility complex (Mhc) of cattle is characterised by multiple polymorphic loci that can vary in number between haplotypes. Previous analysis of the second exon sequences derived from genomic BoLA DQA3 genes identified two distinct families, DQA3*01 and DQA3*02. In this report, we describe the nucleotide and predicted amino acid sequences of the entire coding region of three transcribed BoLA DQA3 genes representing each of these families. These data provide additional evidence that the BoLA DQA3 locus is distinct from BoLA DQA1 and BoLA DQA2. In addition, the amino acid sequence of DQA3 genes from the two families is shown to differ by 35 out of the 254 amino acids. Putative locus-specific amino acid sequence motifs within the transmembrane and intracytoplasmic domains of DQA genes are shown to differ between the DQA3*01 and DQA3*02 genes. Phylogenetic analysis reveals a genetic distance that is considerably larger than that seen between orthologous Mhc allelic families. These data are consistent with either an extremely divergent family of DQA3 genes or an allele at an additional BoLA DQA4 locus.