Neuroprotective effect of 4-hydroxybenzyl alcohol against transient focal cerebral ischemia via anti-apoptosis in rats
Neuroprotective effect of 4-hydroxybenzyl alcohol against transient focal cerebral ischemia via anti-apoptosis in rats
复制标题
4-羟基苯甲醇通过抗细胞凋亡对大鼠短暂性局灶性脑缺血的神经保护作用
DOI：
10.1016/j.brainres.2009.10.037
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发表时间：
2010-01-13
期刊：
影响因子：
2.9
通讯作者：
Zhao, Yong
中科院分区：
文献类型：
--
作者：
Yu, Shan-Shan;Zhao, Jing;Zhao, Yong
4-4-羟基苯甲醇（4-HBA）是天麻（Gastrodia elata Blume）的主要酚类活性成分之一，具有抗中枢和外周神经系统疾病的作用。本研究采用大鼠大脑中动脉闭塞（MCAO）致局灶性脑缺血模型，于缺血前30 min给予4-HBA（25，50 mg/kg），观察4-HBA对缺血再灌注大鼠脑组织的保护作用。在50 mg/kg 4-HBA治疗下，与缺血再灌注值相比，总梗死体积（100.76+/-2.90 mm（3））、皮质梗死体积（64.91 +/-1.46 mm（3））和皮质下梗死体积（38.77+/-2.78 mm（3））显著降低。4-HBA组的神经学评价和Nissl染色与未治疗的缺血组相比显著改善。4-HBA处理组TUNEL阳性细胞明显减少。免疫荧光染色分析和Western免疫印迹结果表明，4-HBA可增加Bcl-2的表达，抑制caspase-3的活化，最终抑制细胞凋亡。提示4-HBA可减轻大鼠局灶性脑缺血损伤，其作用机制可能与抑制细胞凋亡有关。(C)2009 Elsevier B. V.保留所有权利。
4-hydroxybenzyl alcohol (4-HBA), one of the major active phenolic constituents of Gastrodia elata Blume, a very important traditional Chinese medicinal herb, has been shown to be an effective agent against the central and peripheral nervous disorders. in this study, we attempted to explore the possible mechanisms underlying the neuroprotection against transient focal cerebral ischemia by 4-HBA.4-HBA (25, 50 mg/kg) was given 30 min before focal ischemia in rats caused by middle cerebral artery occlusion (1 h of occlusion, 24 h of reperfusion). Under the treatment of 50 mg/kg 4-HBA, total (100.76+/-2.90 mm(3)), Cortical (64.91 +/- 1.46 mm(3)), and sub-cortical (38.77+/-2.78 mm(3)) infarct volumes were significantly decreased in comparison to ischemia-reperfusion values. Neurological evaluation and Nissl-staining of the 4-HBA group were improved significantly compared to the untreated ischemia group. TUNEL-positive cells were reduced significantly in 4-HBA treated group. Results of immunofluorescence staining analysis and Western immunoblot indicated that 4-HBA increased the expression of Bcl-2 and inhibited the activation of caspase-3 ultimately inhibiting apoptosis. These results suggested that 4-HBA ameliorated ischemic injury induced by transient focal cerebral ischemia in rats, and this neuroprotective effect may be partly related to attenuate apoptosis pathway. (C) 2009 Elsevier B.V. All rights reserved.