Effects of bone marrow stromal cell injection in an experimental glaucoma model
Effects of bone marrow stromal cell injection in an experimental glaucoma model
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DOI：
10.1016/j.bbrc.2006.03.231
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发表时间：
2006-06-16
影响因子：
3.1
通讯作者：
Yoshimura, Nagahisa
中科院分区：
文献类型：
--
作者：
Yu, Saiyuu;Tanabe, Teruyo;Yoshimura, Nagahisa
我们研究了将骨髓基质细胞（BMSCs）移植到青光眼模型眼玻璃体中是否可以整合到宿主视网膜中，以及它们是否可以拯救视网膜神经节细胞（RGC）免于由高眼压诱导的死亡。通过结扎巩膜外静脉在成年Wistar大鼠的右眼中诱导青光眼。将表达GFP的BMSCs（GFP-BMSCs）注射到对照眼和青光眼眼的玻璃体中。移植后GFP-BMSCs主要沿着内界膜分布，少数细胞整合入神经节细胞层。移植后2周或4周，观察到GFP-BMSCs表达各种营养因子。与PBS注射的青光眼眼相比，BMSC注射的青光眼模型眼显示出较少的RGC数量减少。这项研究表明，骨髓间充质干细胞移植可能是值得作为一种神经保护工具，治疗青光眼。(c)2006年爱思唯尔公司All rights reserved.
We investigated if bone marrow stromal cells (BMSCs) transplanted into the vitreous body of a glaucoma model eye could be integrated in the host retina and also whether they could rescue the retinal ganglion cells (RGCs) from death induced by the elevated intraocular pressure. Glaucoma was induced in the right eye of adult Wistar rats by ligating the episcleral veins. The GFP-expressing BMSCs (GFP-BMSCs) were injected into the vitreous body of both the control and the glaucomatous eyes. After transplantation, GFP-BMSCs were mostly present along with the inner limiting membrane and only a few cells were integrated into the ganglion cell layer. At 2 or 4 weeks after transplantation, GFP-BMSCs were observed to express various trophic factors. The BMSCs injected glaucoma model eyes showed less reduction in the number of RGCs compared to the glaucomatous eyes with PBS injection. This study suggests that BMSC transplantation may be worthy as a neuroprotective tool to treat glaucoma. (c) 2006 Elsevier Inc. All rights reserved.