L-fucose ameliorates the carcinogenic properties of Fusobacterium nucleatum in colorectal cancer.
L-fucose ameliorates the carcinogenic properties of Fusobacterium nucleatum in colorectal cancer.
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L-岩藻糖改善结直肠癌中具核梭杆菌的致癌特性
DOI：
10.3892/ol.2020.12404
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发表时间：
2021-03
期刊：
影响因子：
2.9
通讯作者：
Hou X
中科院分区：
文献类型：
--
作者：
Duan C;Tang X;Wang W;Qian W;Fu X;Deng X;Han C;Hou X
核梭杆菌(FN)被认为是结直肠癌(CRC)的促进因子；然而，只有少数研究对FN的治疗进行了研究。L岩藻糖是一种具有益生潜力的天然单糖。本研究旨在探讨L岩藻糖对FN致癌作用的影响。将FN和FN+L岩藻糖分别作用于人结肠癌细胞株HCT116和SW480。细胞计数试剂盒(CCK-8)、集落形成、Transwell迁移和侵袭及伤口愈合实验分别检测细胞的增殖、迁移和侵袭能力。Western印迹法检测JAK/STAT3信号通路成分和EMT的蛋白水平。CCK-8、集落形成、Transwell和伤口愈合实验结果表明，FN可显著增强HCT116和SW480结肠癌细胞的增殖、迁移和侵袭能力。值得注意的是，在添加L岩藻糖后，这些影响显著逆转。此外，L岩藻糖还能抑制FN的致癌作用，激活STAT3通路和上皮细胞向间充质细胞的转化。综上所述，本研究结果表明，L岩藻糖在体外可以改善FN的致癌特性，因此可能成为菌群相关性结肠癌的一个新的治疗靶点。
Fusobacterium nucleatum (Fn) is considered a promoting factor in colorectal cancer (CRC); however, only a few studies have investigated therapies against Fn. L-fucose is a natural monosaccharide that has prebiotic potential. The present study aimed to investigate the effect of L-fucose on the carcinogenic properties of Fn. The HCT116 and SW480 colon cancer cell lines were treated with Fn and Fn+L-fucose (Fnf), respectively. The Cell Counting Kit-8 (CCK-8), colony formation, Transwell migration and invasion and wound healing assays were performed to assess the proliferative, migratory and invasive abilities of the cells, respectively. Western blot was performed to detect the protein levels of jak/stat3 pathway components and EMT. The results of the CCK-8, colony formation, Transwell and wound healing assays demonstrated that treatment with Fn significantly enhanced the proliferative, migratory and invasive abilities of HCT116 and SW480 colon cancer cells. Notably, these effects were significantly reversed following addition of L-fucose. Furthermore, L-fucose inhibited the carcinogenic properties of Fn to activate the stat3 pathway and epithelial-to-mesenchymal transition. Taken together, the results of the present study suggest that L-fucose ameliorates the carcinogenic properties of Fn in vitro, and thus may serve as a novel therapeutic target for flora-related colon cancer.
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影响因子：
24.5
作者：
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影响因子：
10.5
作者：
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影响因子：
4.9
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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