Natural history of human respiratory syncytial virus inferred from phylogenetic analysis of the attachment (G) glycoprotein with a 60-nucleotide duplication
Natural history of human respiratory syncytial virus inferred from phylogenetic analysis of the attachment (G) glycoprotein with a 60-nucleotide duplication
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DOI：
10.1128/jvi.80.2.975-984.2006
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发表时间：
2006-01-01
影响因子：
5.4
通讯作者：
Melero, JA
中科院分区：
文献类型：
--
作者：
Trento, A;Viegas, M;Melero, JA
在布宜诺斯艾利斯（BA）的呼吸道感染的主动监测计划（1999年至2004年）期间，共鉴定出47份临床样本，其中含有在附着（G）蛋白基因中具有60个核苷酸重复的人呼吸道合胞病毒（HRSV）序列。这种重复类似于之前描述的1999年在布宜诺斯艾利斯分离的其他三种病毒（A.特伦托等人，J.将军·维洛。84：3115-3120，2003）。系统发育分析表明，BA序列的重复共享一个共同的祖先（约1998年）与其他HRSV的G序列在1999年后在世界范围内报道。在早期病毒中，重复的核苷酸序列是前面60个核苷酸的精确拷贝，但在较新的病毒中，重复片段的两个拷贝以明显高于G蛋白基因其他区域的速率积累核苷酸替换。具有重复G片段的病毒的进化显然遵循先前描述的HRSV的总体进化模式，并且该基因型已经取代了布宜诺斯艾利斯和其他地方的其他流行抗原组B基因型。因此，复制的片段代表了一个天然的标签，可以用来跟踪HRSV在前所未有的环境中的传播和演变。我们已经利用这种情况，重新审查HRSV的分子流行病学，并探讨这一重要的人类病原体的自然史。
A total of 47 clinical samples were identified during an active surveillance program of respiratory infections in Buenos Aires (BA) (1999 to 2004) that contained sequences of human respiratory syncytial virus (HRSV) with a 60-nucleotide duplication in the attachment (G) protein gene. This duplication was analogous to that previously described for other three viruses also isolated in Buenos Aires in 1999 (A. Trento et al., J. Gen. Virol. 84:3115-3120, 2003). Phylogenetic analysis indicated that BA sequences with that duplication shared a common ancestor (dated about 1998) with other HRSV G sequences reported worldwide after 1999. The duplicated nucleotide sequence was an exact copy of the preceding 60 nucleotides in early viruses, but both copies of the duplicated segment accumulated nucleotide substitutions in more recent viruses at a rate apparently higher than in other regions of the G protein gene. The evolution of the viruses with the duplicated G segment apparently followed the overall evolutionary pattern previously described for HRSV, and this genotype has replaced other prevailing antigenic group B genotypes in Buenos Aires and other places. Thus, the duplicated segment represents a natural tag that can be used to track the dissemination and evolution of HRSV in an unprecedented setting. We have taken advantage of this situation to reexamine the molecular epidemiology of HRSV and to explore the natural history of this important human pathogen.