ENHANCEMENT OF SKELETAL-MUSCLE REGENERATION
ENHANCEMENT OF SKELETAL-MUSCLE REGENERATION
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DOI：
10.1002/aja.1002010105
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发表时间：
1994-09-01
影响因子：
2.5
通讯作者：
HEINTZ, C
中科院分区：
文献类型：
--
作者：
BISCHOFF, R;HEINTZ, C
本文研究了添加额外的卫星细胞或粉碎肌肉中的可溶性因子对大鼠胫骨肌碎块再生的影响。将肌肉切碎物包裹在人工肌外膜中以防止粘连和细胞从邻近肌肉迁移。再生进行了定量评估的肌肉特异性形式的肌酸激酶电泳测定。在7周的再生期内，对照切碎物表现出三个时期的肌酸激酶活性的变化。活动在第一周迅速下降，然后从1-3周逐渐上升，从3-7周增加得更快。为了增加肌源性细胞的原始补充，从对侧肌肉分离卫星细胞，通过密度梯度离心纯化，并在培养物中扩增3天，然后添加到肌肉碎肉中。添加的细胞导致在整个再生期间肌酸激酶活性的3倍增强。将可溶性肌肉提取物加入胶原蛋白基质中，加入肌肉碎肉中也能刺激再生。在1-3周期间，浸提物加速肌酸激酶增加的速率，超过对照或细胞增强的肉末中观察到的速率，表明浸提物中的因子可能促进血管再生或神经再支配。肌酸激酶的比活性增加与细胞和提取物的再生增强，表明该效果主要增强生肌过程。(C)1994 Wiley-Liss，Inc.
We have studied the effect of adding extra satellite cells or soluble factors from crushed muscle on regeneration of minced fragments from rat tibialis muscle. The muscle mince was wrapped in an artificial epimysium to prevent adhesions and cell immigration from adjacent muscles. Regeneration was quantitatively assessed by electrophoretic determination of the muscle-specific form of creatine kinase. Control minces exhibited three periods of change in creatine kinase activity during a 7-week regeneration period. Activity fell rapidly during the first week, then rose gradually from 1-3 weeks and increased more rapidly from 3-7 weeks. To augment the original complement of myogenic cells, satellite cells were isolated from the contralateral muscle, purified by density gradient centrifugation, and expanded in culture for 3 days before adding to the muscle mince. The added cells resulted in a 3-fold enhancement of creatine kinase activity throughout the regeneration period. Soluble muscle extract incorporated into a collagen matrix also stimulated regeneration when added to muscle mince. The extract accelerated the rate of creatine kinase increase during the 1-3 week period beyond that observed in the control or cell augmented mince, suggesting that factors in the extract may facilitate revascularization or reinnervation. The specific activity of creatine kinase was increased in regenerates augmented with both cells and extract, indicating that the effects enhance primarily myogenic processes. (C) 1994 Wiley-Liss, Inc.