IL-1β Plays an Important Role in Pressure Overload-Induced Atrial Fibrillation in Mice
IL-1β Plays an Important Role in Pressure Overload-Induced Atrial Fibrillation in Mice
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DOI：
10.1248/bpb.b18-00363
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发表时间：
2019-04-01
影响因子：
2
通讯作者：
Hirose, Masamichi
中科院分区：
文献类型：
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作者：
Matsushita, Naoko;Ishida, Nanae;Hirose, Masamichi
高血压是心房颤动（AF）的一种风险，并诱导心脏炎症。最近的证据表明，压力超负荷诱导的心室结构重塑与核苷酸结合寡聚化结构域（NOD）样受体P3（NLRP 3）炎性小体的激活有关，包括一种含有C-末端半胱天冬酶募集结构域（ASC）的凋亡相关斑点样蛋白。我们假设NLRP 3炎性小体是压力超负荷诱导的心房重构中危险信号的初始传感器，导致AF。在ASC（-/-）和白细胞介素-1 β（IL-1 β（-/-））缺陷的小鼠中进行横向主动脉缩窄（TAC）或假手术。术后1周行食管左房短阵起搏30 s诱发房颤，检测IL-1 β、单核细胞趋化蛋白1（MCP-1）、结缔组织生长因子（CTGF）和胶原1基因表达。电短阵起搏诱发的房颤在经导管心房颤动手术的野生型（WT）中（p
Hypertension is one risk for atrial fibrillation (AF) and induces cardiac inflammation. Recent evidence indicates that pressure overload-induced ventricular structural remodeling is associated with the activation of nucleotide binding-oligomerization domain (NOD)-like receptor P3 (NLRP3) inflammasomes, including an apoptosis-associated speck-like protein containing a C-terminal caspase recruitment domain (ASC). We hypothesized that NLRP3 inflammasomes are an initial sensor for danger signals in pressure overload-induced atrial remodeling, leading to AF. Transverse aortic constriction (TAC) or a sham procedure was performed in mice deficient for ASC(-/-) and interleukin-1 beta (IL-1 beta(-/-)). One week after the procedure, electrical left atrial burst pacing from the esophagus was performed for 30s to induce AF. IL-1 beta, monocyte chemotactic protein 1 (MCP-1), connective tissue growth factor (CTGF), and collagen 1 gene expression were also examined. The electrical burst pacing induced AF in TAC-operated wild-type (WT) (p