Assignment of the TCF-4 gene (TCF7L2) to human chromosome band 10q25.3
Assignment of the TCF-4 gene (TCF7L2) to human chromosome band 10q25.3
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DOI：
10.1159/000015534
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发表时间：
2000-01-01
期刊：
影响因子：
--
通讯作者：
Hamelin, R
中科院分区：
文献类型：
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作者：
Duval, A;Busson-Leconiat, M;Hamelin, R
人类TCF7L2基因从CEPH （Centre d’etude du Polymorphisme human）基因组BAC （Bacterial Artificial Chromosome）文库中克隆。经荧光原位杂交（FISH）鉴定，定位于染色体10q25带。3. 在FISH实验中，人类BAC被用作从健康男性的pha刺激血细胞培养中获得的高分辨率中期染色体的探针。杂交信号在红色染色体上显示为黄色斑点（FITC）（碘化丙啶和DAPI反染，允许带识别）。
The human TCF7L2 gene was cloned from the CEPH (Centre d'Etude du Polymorphisme Humain) genomic BAC (Bacterial Artificial Chromosome) library. It was shown by fluorescence in situ hybridization (FISH) that it mapped to chromosome band 10q25. 3. This human BAC was used as a probe in FISH experiments to high resolution metaphase chromosomes obtained from PHA-stimulated blood cell cultures of a healthy male. The hybridization signal appeared as yellow spots (FITC) on red chromosomes (counterstained with propidium iodide and DAPI allowing the banding recognition).