Chimerism after vascularized limb versus bone marrow transplantation.
Chimerism after vascularized limb versus bone marrow transplantation.
复制标题
血管化肢体与骨髓移植后的嵌合现象。
DOI：
10.1055/s-2006-946716
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发表时间：
2006
影响因子：
2.1
通讯作者：
Tung,ThomasH
中科院分区：
文献类型：
--
作者：
Cohen,Michael;Mohanakumar,Thalachallour;Mackinnon,SusanE;Tung,ThomasH
这项研究在小鼠模型中使用定量 PCR 来比较同种异体肢体移植物与相当剂量的骨髓悬浮液诱导嵌合的能力。雌性C57Bl/6小鼠接受带血管的后肢同种异体移植物、相当剂量的5×10 6 供体骨髓细胞或来自雄性Balb/c供体的标准剂量(20×10 6 )骨髓悬浮液。所有接受者均接受基于 CD40 共刺激阻断和 T 细胞耗竭的治疗方案。 Y染色体特异性定量PCR用于测量嵌合状态。大多数同种异体肢体移植受者在 1 周 (3/4) 和 1 个月 (3/4) 后表现出低水平的嵌合状态。大多数5×10 6 骨髓细胞的受体在1周时嵌合水平较低(4/6)，1个月后仅为1/5。 1周(5/5)和1个月(5/6)后，除了一个细胞外，所有20×10 6 细胞的受体都表现出低水平或高水平的嵌合状态。因此，与相当剂量的骨髓悬浮液相比，同种异体肢体移植物的骨髓成分在诱导微嵌合方面更有效。
This study used quantitative PCR in the murine model to compare the ability of a limb allograft vs. a comparable dose of marrow suspension to induce chimerism. Female C57Bl/6 mice received a vascularized hindlimb allograft, a comparable dose of 5× 10 6 donor bone marrow cells, or a standard dose (20× 10 6) of marrow suspension from male Balb/c donors. All recipients were treated with a regimen based on CD40 costimulation blockade and T cell depletion. Y chromosome-specific quantitative PCR was used to measure chimerism. Most recipients of limb allografts demonstrated low levels of chimerism after 1 week (3/4) and 1 month (3/4). Most recipients of 5× 10 6 marrow cells had low levels of chimerism at 1 week (4/6) and only 1/5 after 1 month. All recipients of 20× 10 6 cells except one demonstrated either low or high levels of chimerism after 1 week (5/5) and 1 month (5/6). The marrow component of a limb allograft is thus more effective at inducing microchimerism compared to a comparable dose of bone marrow suspension.