Accumulation and molecular regulation of anthocyanin in purple tumorous stem mustard (Brassica juncea var. tumida Tsen et Lee).
Accumulation and molecular regulation of anthocyanin in purple tumorous stem mustard (Brassica juncea var. tumida Tsen et Lee).
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DOI：
10.1021/jf501790a
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发表时间：
2014-07
影响因子：
6.1
通讯作者：
Q. Xie;Zongli Hu;Yanjie Zhang;Shibing Tian;Zhijin Wang;Zhiping Zhao;Yang Yang-Yang;Guoping Chen
中科院分区：
文献类型：
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作者：
Q. Xie;Zongli Hu;Yanjie Zhang;Shibing Tian;Zhijin Wang;Zhiping Zhao;Yang Yang-Yang;Guoping Chen
茎瘤芥（Brassica juncea var. tumida Tsen et Lee）是亚洲国家经济和营养重要的膳食蔬菜。紫茎瘤芥具有膨大的瘤茎和叶片中丰富的花青素积累。采用高效液相色谱-电喷雾串联质谱（HPLC-ESI-MS/MS）技术从紫茎芥中分离鉴定了20种花色苷。为了研究紫色瘤茎芥菜花青苷产生的调控机制，对紫色和绿色品种叶片中花青苷生物合成和调控基因的表达进行了研究。调控基因BjTT 8和所有生物合成基因在紫色品种中显著上调。此外，BjTT 8和所有结构基因的转录水平，除了BjPAL，都显着高于在黑暗中处理的芽。这些结果表明，BjTT 8的转录激活与大多数花青素生物合成基因的上调有关，以产生紫色茎瘤芥中的花青素。
Tumorous stem mustard (Brassica juncea var. tumida Tsen et Lee) is an economically and nutritionally important dietary vegetable in Asian countries. Purple tumorous stem mustard contains inflated tumorous stem and abundant anthocyanin accumulation in leaves. Here, 20 anthocyanins were separated and identified from the purple tumorous stem mustard by high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS). In order to investigate the regulatory anthocyanin production in purple tumorous stem mustard, the expression of anthocyanin biosynthetic and regulatory genes in leaves from purple and green cultivars were examined. Regulatory gene BjTT8 and all biosynthetic genes were dramatically upregulated in the purple variety. Moreover, the transcript level of BjTT8 and all structural genes, except BjPAL, were all significantly higher in light-treated sprouts than in the dark. These results indicate that transcriptional activation of BjTT8 is associated with upregulation of most anthocyanin biosynthetic genes, to produce anthocyanins in purple tumorous stem mustard.