Effects of Diallyl Sulfide and Zinc on Testicular Steroidogenesis in Cadmium-Treated Male Rats
Effects of Diallyl Sulfide and Zinc on Testicular Steroidogenesis in Cadmium-Treated Male Rats
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DOI：
10.1002/jbt.20247
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发表时间：
2008-01-01
影响因子：
3.6
通讯作者：
Sadik, Nermin A. H.
中科院分区：
文献类型：
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作者：
Sadik, Nermin A. H.
镉（Cd）是影响包括睾丸在内的各种组织和器官的环境污染物之一。已知镉对睾丸的有害影响是生殖细胞变性和睾丸类固醇生成受损。在本研究中，研究了二烯丙基硫醚（DAS）（大蒜中存在的一种含硫挥发性化合物）和锌（Zn）对镉引起的大鼠睾丸毒性的影响。用镉治疗的雄性成年 Wistar 大鼠（2.5 毫克/千克体重，每周 5 次，持续 4 周）显示体重、配对睾丸重量、相对睾丸重量、血清睾酮、黄体生成素、卵泡刺激素、睾丸总抗氧化能力 (TAC) 和蛋白质水平下降。睾丸类固醇生成酶，如 3 β-羟基类固醇脱氢酶 (3 β-HSD) 和 17 β-羟基类固醇脱氢酶 (17 β-HSD)，以及标记酶，如山梨醇脱氢酶 (SDH)、乳酸脱氢酶 (LDH)、酸性磷酸酶 (ACP)、碱性磷酸酶 (ALP) 和 6-磷酸葡萄糖脱氢酶 (G6PD)，显示出显着降低镉暴露后，γ-谷氨酰转移酶的活性显着增加。结果表明，同时使用 DAS 或锌治疗可恢复关键类固醇生成酶、SDH、LDH 和 G6PD，并显着增加睾丸重量。 DAS 恢复了 TAC 水平，并显着增加了睾酮水平和相对睾丸重量。锌恢复睾丸蛋白质水平和体重。可以得出的结论是，镉可能通过影响垂体促性腺激素而导致成年雄性大鼠的睾丸毒性并抑制雄激素的产生，并且同时服用DAS或锌可以防止镉诱导的睾丸毒性。 (C) 2008 Wiley periodicals, Inc. J Biochem Mol Toxicol 22:345-353, 2008；在线发表于 Wiley InterScience (www.interscience.wiley.com)。 DOI 10:1002/jbt.20247
Cadmium (Cd) is one of the environmental pollutants that affect various tissues and organs including testis. Harmful effect of cadmium on testis is known to be germ cell degeneration and impairment of testicular steroidogenesis. In the present study, the effect of diallyl sulfide (DAS), a sulfur-containing volatile compound present in garlic, and zinc (Zn) was investigated on cadmium-induced testicular toxicity in rats. Male adult Wistar rats treated with cadmium (2.5 mg/kg body wt, five times a week for 4 weeks) showed decreased body weight, paired testicular weight, relative testicular weight, serum testosterone, luteinizing hormone, follicle-stimulating hormone, and testicular total antioxidant capacity (TAC) and protein levels. Testicular steroidogenic enzymes, such as 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) and 17 beta-hydroxysteroid dehydrogenase (17 beta-HSD), and marker enzymes, such as sorbitol dehydrogenase (SDH), lactate dehydrogenase (LDH), acid phosphatase (ACP), alkaline phosphatase (ALP), and glucose-6-phosphate dehydrogenase (G6PD), showed a significant decrease in activities whereas that of gamma-glutamyl transferase was significantly increased after cadmium exposure. The results have revealed that concurrent treatment with DAS or zinc restored key steroidogenic enzymes, SDH, LDH, and G6PD and increased testicular weight significantly. DAS restored the TAC level and increased testosterone level and relative testicular weight significantly. Zinc restored testicular protein level and body weight. It can be concluded that cadmium causes testicular toxicity and inhibits androgen production in adult male rats probably by affecting pituitary gonadotrophins and that concurrent administration of DAS or zinc provides protection against cadmium-induced testicular toxicity. (C) 2008 Wiley Periodicals, Inc. J Biochem Mol Toxicol 22:345-353, 2008; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10:1002/jbt.20247