Keratin 19 as a biochemical marker of skin stem cells in vivo and in vitro: keratin 19 expressing cells are differentially localized in function of anatomic sites, and their number varies with donor age and culture stage.
Keratin 19 as a biochemical marker of skin stem cells in vivo and in vitro: keratin 19 expressing cells are differentially localized in function of anatomic sites, and their number varies with donor age and culture stage.
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发表时间：
1996-05
影响因子：
4
通讯作者：
M. Michel;Natalie Török;M. Godbout;Marc Lussier;Pierrette Gaudreau;André Royal;Lucie Germain
中科院分区：
文献类型：
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作者：
M. Michel;Natalie Török;M. Godbout;Marc Lussier;Pierrette Gaudreau;André Royal;Lucie Germain
本研究旨在评估角蛋白19（K19）作为皮肤干细胞的生化标志物，以解决皮肤生物学领域中有关这些细胞的一些长期存在的问题。我们首先使用完善的小鼠模型，使我们能够将皮肤干细胞鉴定为[3H]胸腺嘧啶标记保留细胞。针对K19的合成肽产生定点抗体。在免疫印迹上，它与40 kDa蛋白（K19）特异性反应。用免疫组化法标记小鼠皮肤外根鞘隆起区。双标记显示K19阳性细胞也是[3H]胸腺嘧啶标记保留细胞，提示K19是毛囊皮肤干细胞的标记物。K19表达随后被用于研究小鼠和人皮肤干细胞中的变化作为身体部位、供体年龄和培养时间的函数。K19在毛囊中表达，在有毛部位的毛囊间表皮中不表达（除了一些共表达K18的默克尔细胞）。相反，在无毛部位，K19阳性细胞位于深表皮网脊。K19表达细胞还含有高水平的α 3 β 1整联蛋白。新生儿包皮中K19阳性细胞的比例高于老年包皮。这与角质形成细胞培养寿命随供体年龄的变化有关。此外，它可以解释临床观察，儿童愈合速度比成人快。总之，K19在皮肤中的表达提供了一个额外的工具，允许进一步表征皮肤干细胞在正常和病理条件下原位和体外。
This study was undertaken to evaluate keratin 19 (K19) as a biochemical marker for skin stem cells in order to address some long standing questions concerning these cells in the field of cutaneous biology. We first used the well-established mouse model enabling us to identify skin stem cells as [3H]thymidine-label-retaining cells. A site directed antibody was raised against a synthetic peptide of K19. It reacted specifically with a 40 kDa protein (K19) on immunoblotting. It labelled the bulge area of the outer root sheath on mouse skin by immunohistochemistry. Double-labelling revealed that K19-positive-cells were also [3H]thymidine-label-retaining cells, suggesting that K19 is a marker for skin stem cells of hair follicles. K19-expression was then used to investigate the variation in mouse and human skin stem cells as a function of body site, donor age and culture time. K19 was expressed in the hair follicle and absent from the interfollicular epidermis at hairy sites (except for some K18 coexpressing Merkel cells). In contrast, at glabrous sites, K19-positive-cells were in deep epidermal rete ridges. K19 expressing cells also contained high levels of alpha 3 beta 1 integrin. The proportion of K19-positive-cells was greater in newborn than older foreskins. This correlated with keratinocyte culture lifespan variation with donor age. Moreover, it could explain clinical observations that children heal faster than adults. In conclusion, K19 expression in skin provides an additional tool to allow further characterization of skin stem cells under normal and pathological conditions in situ and in vitro.