SECONDARY EFFECTS IN AQUEOUS SIZE EXCLUSION CHROMATOGRAPHY OF SODIUM POLY(STYRENESULFONATE) COMPOUNDS
SECONDARY EFFECTS IN AQUEOUS SIZE EXCLUSION CHROMATOGRAPHY OF SODIUM POLY(STYRENESULFONATE) COMPOUNDS
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DOI：
10.1021/ac00181a006
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发表时间：
1989-03-15
影响因子：
7.4
通讯作者：
MORI, S
中科院分区：
文献类型：
--
作者：
MORI, S
研究了聚苯乙烯磺酸钠（NaPSS）化合物在几种市售色谱柱上在不同离子强度的磷酸盐缓冲液（pH 8.0）中的洗脱行为。本文提出了一种阐明离子型聚合物保留行为的方法，包括在不同离子强度下建立离子型和非离子型聚合物的通用标准曲线，比较不同离子强度下离子型聚合物的色谱图，绘制离子型和非离子型聚合物的通用标准曲线的分歧，以及离子排阻体积与离子强度的关系。NaPSS在多孔玻璃柱上的通用标准曲线与普鲁兰多糖的通用标准曲线一致，但在其他柱上的通用标准曲线随离子强度的变化而超过普鲁兰多糖的标准曲线。离子排阻是对多孔玻璃柱的唯一次要影响，并且该影响在高离子强度下被抑制，从而实现理想的尺寸排阻色谱（SEC）。在其他柱上，NaPSS的早期洗脱受离子排斥效应的影响，随着离子强度的增加，NaPSS与底物之间的疏水相互作用变得明显。NaPSS的保留体积受三种效应的影响：尺寸排阻、离子排阻和疏水相互作用。理想的SEC是不可能实现的。
Elution behavior of sodium poly(styrenesulfonate) (NaPSS) compounds on several commercially available columns was studied at varying ionic strengths of phosphate buffer at pH 8.0. A procedure to elucidate the retention behavior of ionic polymers is proposed, which consists of construction of universal calibration curves for both ionic and nonionic polymers at various ionic strengths, comparison of chromatograms of ionic polymers at various ionic strengths, plotting the divergence of universal calibration curves for both ionic and nonionic polymers, and ion-exclusion volume as a function of ionic strength. Universal calibration curves of NaPSS on porous glass columns converged to that of pullulan, but those on other columns went over the pullulan curve with the ionic strength. Ion exclusion is the only secondary effect on porous glass columns and this effect is suppressed at high ionic strength, achieving ideal size exclusion chromatography (SEC). On other columns tested, early elution of NaPSS is governed by ion-exclusion effect, and with increasing the ionic strength, hydrophobic interactions between NaPSS and substrates become remarkable. Retention volume of NaPSS is governed by the compromise among three effects: size-exclusion, ion-exclusion, and hydrophobic interactions. Ideal SEC cannot be expected.