A facile and sensitive detection of pathogenic bacteria using magnetic nanoparticles and optical nanocrystal probes
A facile and sensitive detection of pathogenic bacteria using magnetic nanoparticles and optical nanocrystal probes
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DOI：
10.1039/c2an35369e
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发表时间：
2012-01-01
期刊：
影响因子：
4.2
通讯作者：
Jeon, Sangmin
中科院分区：
文献类型：
--
作者：
Joo, Jinmyoung;Yim, Changyong;Jeon, Sangmin
我们报告了一种简便、灵敏的病原菌检测方法。采用抗体偶联磁性纳米颗粒（MNPs）捕获牛奶中的沙门氏菌，外加磁场将其从分析样品中分离出来。将mnp -沙门氏菌复合物重新分散在缓冲溶液中，然后暴露于抗体固定的TiO2纳米晶体（TNs）中，该纳米晶体吸收紫外线。磁性分离mnp -沙门氏菌-TN配合物后，得到未结合TN溶液的紫外-可见吸收光谱。由于光吸收强度与沙门氏菌浓度成反比，该方法对低浓度的沙门氏菌具有较高的敏感性。牛奶中沙门氏菌的检出限大于100 cfu mL（-1）。
We report a facile and sensitive analytical method for the detection of pathogenic bacteria. Salmonella bacteria in milk were captured by antibody-conjugated magnetic nanoparticles (MNPs) and separated from analyte samples by applying an external magnetic field. The MNP-Salmonella complexes were re-dispersed in a buffer solution then exposed to antibody-immobilized TiO2 nanocrystals (TNs), which absorb UV light. After magnetically separating the MNP-Salmonella-TN complexes from solution, the UV-Vis absorption spectrum of the unbound TN solution was obtained. Because the light absorption intensity was reversely proportional to the Salmonella concentration, the assay exhibited high sensitivity toward low concentrations of Salmonella bacteria. The detection limit of Salmonella in milk was found to be more than 100 cfu mL(-1).