Replication in UV-irradiated Caenorhabditis elegans embryos
Replication in UV-irradiated Caenorhabditis elegans embryos
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DOI：
10.1111/j.1751-1097.1996.tb03012.x
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发表时间：
1996-02-01
影响因子：
3.3
通讯作者：
Hartman, PS
中科院分区：
文献类型：
--
作者：
Jones, CA;Hartman, PS
即使在暴露于大量紫外线辐射后，野生型（但不是 rad 突变体）秀丽隐杆线虫胚胎的复制仍在继续。阐明这种“抗损伤”DNA 合成的机制是很有意义的。在这项研究中，使用电子显微镜检查了未照射和紫外线照射的野生型胚胎的 DNA。紫外辐射的大能量密度（180 J m(-2)）对野生型胚胎中的复制气泡大小或气泡之间的距离几乎没有影响，表明抗损伤DNA合成并没有严重异常。相反，紫外线照射显着降低了切除修复缺陷的 rad-3 胚胎中气泡之间的中心距离。这表明在 rad-3 胚胎中紫外线照射后观察到的 DNA 合成减少主要是由于 DNA 合成伸长受阻。
Replication continues in wild-type (but not rad mutant) Caenorhabditis elegans embryos even after exposure to massive fluences of UV radiation. It is of interest to elucidate the mechanism(s) for this ''damage-resistant'' DNA synthesis. In this study, DNA from unirradiated and UV-irradiated wild-type embryos was examined using the electron microscope. Large fluences of UV radiation (180 J m(-2)) had little effect on either replication bubble size or distances between bubbles in wild-type embryos, indicating that the damage-resistant DNA synthesis was not grossly aberrant. Conversely, UV irradiation significantly decreased center-to-center distances between bubbles in excision-repair-deficient rad-3 embryos. This suggests that the decreased DNA synthesis observed after UV irradiation in rad-3 embryos is due largely to blockage of elongation of DNA synthesis.