Correlations of PD-L1 gene polymorphisms with susceptibility and prognosis in hepatocellular carcinoma in a Chinese Han population
Correlations of PD-L1 gene polymorphisms with susceptibility and prognosis in hepatocellular carcinoma in a Chinese Han population
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DOI：
10.1016/j.gene.2018.06.069
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发表时间：
2018-10-20
期刊：
影响因子：
3.5
通讯作者：
Yang, Zhuying
中科院分区：
文献类型：
--
作者：
Xie, Qigui;Chen, Zhanlei;Yang, Zhuying
目的：本研究旨在探讨PD-L1基因多态性对中国汉族人群肝细胞癌（HCC）易感性和预后的影响。PD-L1基因的4个单核苷酸多态性（SNP），包括rs 2297136（C > T）、rs 4143815（C > G）、应用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术检测225例HCC患者和200例健康对照者的rs 2890658（A > C）和rs 17718883（C > G结果：rs 2297136（C > T）SNP TT（p = 0.03）和rs 4143815（C > G）SNP GG基因型（p < 0.001）与HCC发病风险显著增加相关。在rs 2890658（A > C）SNP与HCC风险之间没有发现关联，并且在具有rs 17718883 SNP CG + GG基因型的个体中，该风险显著降低（p < 0.001）。rs 2297136（C > T）SNP CC + CT基因型、rs 4143815（C > G）CC基因型和rs 2890658（A > C）AA基因型与其对应的等位基因型相比与总生存率增加相关（p < 0.001）。rs 2890658（A > C）SNP对HCC的风险和预后无影响（p > 0.05）。我们的研究结果表明，三个功能多态性PD-L1基因的rs 2297136、rs 4143815和rs 17718883与HCC风险和预后相关，提示PD-L1多态性的遗传变异可能是预测HCC风险和发展的可能预后标志物。需要从更多样化的种族人群中进行更大规模的前瞻性研究来验证这些发现。
Aims: This study was performed to investigate the effect of PD-L1 polymorphisms on the susceptibility and prognosis of hepatocellular carcinoma (HCC) in a Chinese Han population.Methods: Four single nucleotide polymorphisms (SNPs) of the PD-L1 gene, including rs2297136 (C > T), rs4143815 (C > G), rs2890658 (A > C) and rs17718883 (C > G) were examined in 225 HCC patients and 200 healthy controls using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method.Results: Data revealed that the rs2297136 (C > T) SNP TT (p = 0.03) and rs4143815 (C > G) SNP GG genotypes (p < 0.001) were associated with significantly increased risks of HCC. No association was found between rs2890658 (A > C) SNP and HCC risk and this risk was significantly decreased in individuals with the rs17718883 SNP CG + GG genotype (p < 0.001). The rs2297136 (C > T) SNP CC + CT genotypes, the rs4143815 (C > G) CC genotype and the rs2890658 (A > C) AA genotype were associated with increased overall survival compared to their counterpart allelic genotypes (p < 0.001). The rs2890658 (A > C) SNP had no impact on the risk and prognosis of HCC (p > 0.05).Conclusions: Our results indicated that three functional polymorphisms (rs2297136, rs4143815 and rs17718883) of the PD-L1 gene were associated with HCC risk and prognosis, suggesting that genetic variants of PD-L1 polymorphisms might be a possible prognostic marker for the prediction of HCC risk and development. Validation by a larger prospective study from a more diverse ethnic population is needed to confirm these findings.